BIOTECHNOLOGY RESOURCE DIGITAL ELECTRO-OPTICAL IMAGING

Project: Research project

Description

Our objective is to establish a Biotechnology resource for Digital
Imaging Technology Development. Its mission is to facilitate the
development and application of emerging optical quantitation
technologies for research in molecular cell biology. The molecular
dynamics of vital processes in living cells can be revealed and
measured with the help of electro-optical microscopic image
acquisition, photon counting quantitation of weak molecular
fluorescence, and computerized digital image processing and storage
with spatial and temporal transform analysis. The resource is
intended to accommodate the invention, development, design,
construction, improvement, maintenance, and application of
quantitative optical techniques specifically designed to provide
powerful probes of molecular dynamics in living cells. Systems are
to be developed for quantitative fluorescence micro-image
acquisition at faint (Photon counting) levels and for fast
(millisecond) acquisition of brighter transient images with digital
image analysis and processing. Systematic quantitation of receptor
dynamics, cell motion, spatial evolution of messenger ion activity,
membrane potential, pH and receptor distributions and individual
receptor molecular motions are achieved at light microscope
resolution. New developments in dynamic image acquisition and
processing, utilization of chemical indicators, 3D image
acquisition, quantitative confocal microscopy and image processing,
time-resolved fluorescence imaging and two photon excitation are
included in the core research. Collaborative research on cellular
biophysics is to be accommodated from the outset; it provides the
best practical test of new technologies. Training functions will
rely importantly on internships, extended hands-on experience of
visiting students and of scientists who establish the learned
techniques in their home laboratories. We aim to convey
capabilities in instrumentation synthesis as well as experience
with complex new technology. Dissemination pathways will include
technology transfer to instrumentation industry as well as directly
to users of the new technologies. The resource is intended to
provide service in three distinct patterns from the beginning:
Local users of current imaging technologies are accommodated in a
new facility to be provided by the Biotechnology Program.
Explorations of emerging new technologies and certain special
problems are to be accommodated within the resource facility as it
develops. A service function is expected to continue informal
consulting service and provision of pilot facilities for the new
technologies at all levels.
StatusFinished
Effective start/end date9/30/888/31/03

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Photons
Technology
spheroids
photons
resources
Dihematoporphyrin Ether
tumors
NAD
Biotechnology
microscopy
biotechnology
mitochondria
Research
cells
Spatio-Temporal Analysis
proteins
image processing
chemical indicators
emerging
acquisition