MECHANISM OF OUTER HAIR CELL MOTILITY

  • Hallworth, Richard J., (PI)

Project: Research project

Description

The long-term objective of these studies is to elucidate the mechanism
of fast outer hair cell (OHC) motility. The results will be of central
interest in basic studies of cochlear mechanism, because they will
delineate the capabilities of OHC motility for energy input to the
cochlea. This is a necessary precondition for serious theoretical and
experimental examination of the role of OHC motility in cochlear function
and pathology. An important secondary benefit of these studies is their
contribution to cell biology about an extraordinarily fast motility
mechanism, one that is unlike anything seen elsewhere to date. Five experimental studies are proposed. Three are designed to test
concepts of the mechanism that have been derived from earlier studies and
that have been formulated into a comprehensive mathematical model. In
this model, motility is thought to be driven by a large number of
independently-acting motor molecules located in the membrane of the
cell's lateral wall. These "motors" are thought to change conformation
in a stochastic voltage-dependent manner. One study will look for
evidence of motor conformation changes in the noise statistics of the
cell's average length. Another will examine the kinetics of motor
activation at speeds not previously technically possible. The motor is
believed to act antiphasically in the axial and radial directions. The
third proposed study will examine the consistency of this action. A
fourth study will determine the force generating capability of the OHC
under load. Finally, in a fifth study, OHC motility will be used as an
assay to determine the somatic location of voltage-dependent
conductances. In addition, the model studies will be extended and new
alternative model schemes examined. The model studies will proceed in
parallel with and will complement the physiological studies.
StatusFinished
Effective start/end date7/1/9311/30/05

Funding

  • National Institutes of Health: $194,974.00
  • National Institutes of Health: $113,655.00
  • National Institutes of Health: $101,979.00
  • National Institutes of Health: $189,286.00
  • National Institutes of Health: $47,697.00
  • National Institutes of Health: $206,836.00
  • National Institutes of Health: $247,223.00
  • National Institutes of Health
  • National Institutes of Health: $200,805.00
  • National Institutes of Health

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Outer Auditory Hair Cells
locomotion
hair
Cell Movement
Cochlea
Acoustics
pathology
electric potential
Electric Impedance
complement
mathematical models
Stretchers
examination
statistics
Organ of Corti
membranes
Sensory Receptor Cells
kinetics