κ-opioid receptor inhibition of calcium oscillations in spinal cord neurons

Lakshmi Kelamangalath, Shashank M. Dravid, Joju George, Jane V. Aldrich, Thomas F. Murray

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mouse embryonic spinal cord neurons in culture exhibit spontaneous calcium oscillations from day in vitro (DIV) 6 through DIV 10. Such spontaneous activity in developing spinal cord contributes to maturation of synapses and development of pattern-generating circuits. Here we demonstrate that these calcium oscillations are regulated by κ opioid receptors (KORs). The κ opioid agonist dynorphin (Dyn)-A (1-13) suppressed calcium oscillations in a concentration-dependent manner, and both the nonselective opioid antagonist naloxone and the κ-selective blocker norbinal-torphimine eliminated this effect. The KOR-selective agonist (+)-(5α,7α,8β)-N-methyl-N-[7- (1-pyrrolidinyl)-1-oxaspiro[4.5]dec-8-yl]-benzeneacetamide (U69593) mimicked the effect of Dyn-A (1-13) on calcium oscillations. A κ-specific peptide antagonist, zyklophin, was also able to prevent the suppression of calcium oscillations caused by Dyn-A (1-13). These spontaneous calcium oscillations were blocked by 1 μM tetrodotoxin, indicating that they are action potential-dependent. Although the L-type voltage-gated calcium channel blocker nifedipine did not suppress calcium oscillations, the N-type calcium channel blocker ω-conotoxin inhibited this spontaneous response. Blockers of ionotropic glutamate receptors, 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo(f) quinoxaline and dizocilpine maleate (MK-801), also suppressed calcium oscillations, revealing a dependence on glutamate-mediated signaling. Finally, we have demonstrated expression of KORs in glutamatergic spinal neurons and localization in a presynaptic compartment, consistent with previous reports of KOR-mediated inhibition of glutamate release. The KOR-mediated inhibition of spontaneous calcium oscillations may therefore be a consequence of presynaptic inhibition of glutamate release.

Original languageEnglish
Pages (from-to)1061-1071
Number of pages11
JournalMolecular Pharmacology
Volume79
Issue number6
DOIs
StatePublished - Jun 2011

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Calcium Signaling
Opioid Receptors
Spinal Cord
Neurons
Glutamic Acid
Dizocilpine Maleate
Calcium Channel Blockers
Benzeneacetamides
N-Type Calcium Channels
Conotoxins
Ionotropic Glutamate Receptors
Quinoxalines
Narcotic Antagonists
Tetrodotoxin
Nifedipine
Naloxone
Synapses
Opioid Analgesics
Action Potentials
Peptides

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Molecular Medicine

Cite this

κ-opioid receptor inhibition of calcium oscillations in spinal cord neurons. / Kelamangalath, Lakshmi; Dravid, Shashank M.; George, Joju; Aldrich, Jane V.; Murray, Thomas F.

In: Molecular Pharmacology, Vol. 79, No. 6, 06.2011, p. 1061-1071.

Research output: Contribution to journalArticle

Kelamangalath, Lakshmi ; Dravid, Shashank M. ; George, Joju ; Aldrich, Jane V. ; Murray, Thomas F. / κ-opioid receptor inhibition of calcium oscillations in spinal cord neurons. In: Molecular Pharmacology. 2011 ; Vol. 79, No. 6. pp. 1061-1071.
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