Abstract
1. 2,3,7,8-Tetrachlorodibenzo-ρdioxin (TCCD)-induced lipid peroxidation has previously been demonstrated by assessing the hepatic content of thiobarbituric acid reactive substances (TBARS) as well as the NADPH-dependent microsomal formation of TBARS as well as the NADPH-dependent microsomal formation of TBARS using malondialde-hyde as the standard. 2. Changes in membrane fluidity as a result of lipid peroxidation may occur. Therefore the dose- and time-dependent effects of TCDD on lipid peroxidation in mitochondrial, microsomal, and plasma membranes, and changes in membrane fluidity in these subcellular fractions, were examined. Animals were treated with either 50 or 100 μg TCDD/kg orally, and killed 3, 6, or 9 days post-treatment. 3. Time-dependent increases occurred in TBARS content and formation following TCDD administration for all three membranes. Similar results were observed after 50 and 100 μg TCDD/kg. 4. Following TCDD administration, fluorescence polarization measurements as determined by the fluorescence polarization (r) and anisotropy parameter (a.p.) values demonstrated significant decreases in membrane fluidity in all membrane fractions, indicative of membrane structural alterations. 5. Excellent inverse correlations between lipid peroxidation and membrane fluidity were observed. Thus, decreased membrane fluidity and increased membrane damage may contribute to the toxic manifestations of TCDD as a consequence of an oxidative stress.
| Original language | English |
|---|---|
| Pages (from-to) | 979-988 |
| Number of pages | 10 |
| Journal | Xenobiotica |
| Volume | 20 |
| Issue number | 9 |
| DOIs | |
| State | Published - 1990 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Toxicology
- Biochemistry
- Health, Toxicology and Mutagenesis
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced decrease in the fluidity of rat liver membranes. / Alsharif, Naser Z.; Grandjean, C. J.; Murray, W. J.; Stohs, S. J.
In: Xenobiotica, Vol. 20, No. 9, 1990, p. 979-988.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced decrease in the fluidity of rat liver membranes
AU - Alsharif, Naser Z.
AU - Grandjean, C. J.
AU - Murray, W. J.
AU - Stohs, S. J.
PY - 1990
Y1 - 1990
N2 - 1. 2,3,7,8-Tetrachlorodibenzo-ρdioxin (TCCD)-induced lipid peroxidation has previously been demonstrated by assessing the hepatic content of thiobarbituric acid reactive substances (TBARS) as well as the NADPH-dependent microsomal formation of TBARS as well as the NADPH-dependent microsomal formation of TBARS using malondialde-hyde as the standard. 2. Changes in membrane fluidity as a result of lipid peroxidation may occur. Therefore the dose- and time-dependent effects of TCDD on lipid peroxidation in mitochondrial, microsomal, and plasma membranes, and changes in membrane fluidity in these subcellular fractions, were examined. Animals were treated with either 50 or 100 μg TCDD/kg orally, and killed 3, 6, or 9 days post-treatment. 3. Time-dependent increases occurred in TBARS content and formation following TCDD administration for all three membranes. Similar results were observed after 50 and 100 μg TCDD/kg. 4. Following TCDD administration, fluorescence polarization measurements as determined by the fluorescence polarization (r) and anisotropy parameter (a.p.) values demonstrated significant decreases in membrane fluidity in all membrane fractions, indicative of membrane structural alterations. 5. Excellent inverse correlations between lipid peroxidation and membrane fluidity were observed. Thus, decreased membrane fluidity and increased membrane damage may contribute to the toxic manifestations of TCDD as a consequence of an oxidative stress.
AB - 1. 2,3,7,8-Tetrachlorodibenzo-ρdioxin (TCCD)-induced lipid peroxidation has previously been demonstrated by assessing the hepatic content of thiobarbituric acid reactive substances (TBARS) as well as the NADPH-dependent microsomal formation of TBARS as well as the NADPH-dependent microsomal formation of TBARS using malondialde-hyde as the standard. 2. Changes in membrane fluidity as a result of lipid peroxidation may occur. Therefore the dose- and time-dependent effects of TCDD on lipid peroxidation in mitochondrial, microsomal, and plasma membranes, and changes in membrane fluidity in these subcellular fractions, were examined. Animals were treated with either 50 or 100 μg TCDD/kg orally, and killed 3, 6, or 9 days post-treatment. 3. Time-dependent increases occurred in TBARS content and formation following TCDD administration for all three membranes. Similar results were observed after 50 and 100 μg TCDD/kg. 4. Following TCDD administration, fluorescence polarization measurements as determined by the fluorescence polarization (r) and anisotropy parameter (a.p.) values demonstrated significant decreases in membrane fluidity in all membrane fractions, indicative of membrane structural alterations. 5. Excellent inverse correlations between lipid peroxidation and membrane fluidity were observed. Thus, decreased membrane fluidity and increased membrane damage may contribute to the toxic manifestations of TCDD as a consequence of an oxidative stress.
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U2 - 10.3109/00498259009046913
DO - 10.3109/00498259009046913
M3 - Article
VL - 20
SP - 979
EP - 988
JO - Xenobiotica
JF - Xenobiotica
SN - 0049-8254
IS - 9
ER -