2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced decrease in the fluidity of rat liver membranes

Naser Z. Alsharif, C. J. Grandjean, W. J. Murray, S. J. Stohs

Research output: Contribution to journalArticle

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Abstract

1. 2,3,7,8-Tetrachlorodibenzo-ρdioxin (TCCD)-induced lipid peroxidation has previously been demonstrated by assessing the hepatic content of thiobarbituric acid reactive substances (TBARS) as well as the NADPH-dependent microsomal formation of TBARS as well as the NADPH-dependent microsomal formation of TBARS using malondialde-hyde as the standard. 2. Changes in membrane fluidity as a result of lipid peroxidation may occur. Therefore the dose- and time-dependent effects of TCDD on lipid peroxidation in mitochondrial, microsomal, and plasma membranes, and changes in membrane fluidity in these subcellular fractions, were examined. Animals were treated with either 50 or 100 μg TCDD/kg orally, and killed 3, 6, or 9 days post-treatment. 3. Time-dependent increases occurred in TBARS content and formation following TCDD administration for all three membranes. Similar results were observed after 50 and 100 μg TCDD/kg. 4. Following TCDD administration, fluorescence polarization measurements as determined by the fluorescence polarization (r) and anisotropy parameter (a.p.) values demonstrated significant decreases in membrane fluidity in all membrane fractions, indicative of membrane structural alterations. 5. Excellent inverse correlations between lipid peroxidation and membrane fluidity were observed. Thus, decreased membrane fluidity and increased membrane damage may contribute to the toxic manifestations of TCDD as a consequence of an oxidative stress.

Original languageEnglish
Pages (from-to)979-988
Number of pages10
JournalXenobiotica
Volume20
Issue number9
DOIs
StatePublished - 1990

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Fluidity
Membrane Fluidity
Liver
Rats
Thiobarbituric Acid Reactive Substances
Membranes
Lipid Peroxidation
Fluorescence Polarization
NADP
Lipids
Dioxins
Subcellular Fractions
Poisons
Mitochondrial Membranes
Fluorescence
Polarization
1,4-dioxin
Polychlorinated Dibenzodioxins
Oxidative Stress
Oxidative stress

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced decrease in the fluidity of rat liver membranes. / Alsharif, Naser Z.; Grandjean, C. J.; Murray, W. J.; Stohs, S. J.

In: Xenobiotica, Vol. 20, No. 9, 1990, p. 979-988.

Research output: Contribution to journalArticle

Alsharif, Naser Z. ; Grandjean, C. J. ; Murray, W. J. ; Stohs, S. J. / 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced decrease in the fluidity of rat liver membranes. In: Xenobiotica. 1990 ; Vol. 20, No. 9. pp. 979-988.
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