A paper microfluidics experiment has been developed for the undergraduate analytical chemistry laboratory. In this laboratory experiment, students fabricated paper-based microfluidic devices and prepared the device for simultaneous colorimetric glucose and protein measurement. Students were given an "unknown" synthetic urine sample and a set of diagnosis protocols. The students prepared glucose and protein standards and analyzed the urine sample for glucose and protein content. The reagents changed color in the presence of glucose and protein in the urine sample. Students visually compared the extent of the color changes in the unknown to color changes from standard solutions. From these colorimetric results, students recommended whether the patient should undergo further testing.