Abstract
A rapid and sensitive liquid-chromatographic method was developed to quantify the release of tobramycin from polymeric drug delivery systems in vitro. Pre-column derivatization of tobramycin and kanamycin B sulphate (internal standard) was carried out with 2,4,6-trinitrobenzenesulphonic acid. The sample volume required was only 50 μl. The chromatographic separation was achieved on an octyl reversed-phase column with UV detection at 340 nm. This isocratic method was performed at ambient temperature and required only 8 min of chromatography time. The standard curves were linear over the concentration range 0.50-50.0 mg l-1. Inter-day and intra-day relative standard deviations ranged from 3.6 to 9.3% and from 1.6 to 6.8%, respectively. The assay method was used to determine the tobramycin content in different pharmaceutical formulations and to study the stability of the drug both in the solid-state and in solution.
| Original language | English |
|---|---|
| Pages (from-to) | 237-245 |
| Number of pages | 9 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmaceutical Science
Cite this
A liquid-chromatographic method for the determination of tobramycin. / Dash, Alekha K.; Suryanarayanan, R.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 9, No. 3, 1991, p. 237-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A liquid-chromatographic method for the determination of tobramycin
AU - Dash, Alekha K.
AU - Suryanarayanan, R.
PY - 1991
Y1 - 1991
N2 - A rapid and sensitive liquid-chromatographic method was developed to quantify the release of tobramycin from polymeric drug delivery systems in vitro. Pre-column derivatization of tobramycin and kanamycin B sulphate (internal standard) was carried out with 2,4,6-trinitrobenzenesulphonic acid. The sample volume required was only 50 μl. The chromatographic separation was achieved on an octyl reversed-phase column with UV detection at 340 nm. This isocratic method was performed at ambient temperature and required only 8 min of chromatography time. The standard curves were linear over the concentration range 0.50-50.0 mg l-1. Inter-day and intra-day relative standard deviations ranged from 3.6 to 9.3% and from 1.6 to 6.8%, respectively. The assay method was used to determine the tobramycin content in different pharmaceutical formulations and to study the stability of the drug both in the solid-state and in solution.
AB - A rapid and sensitive liquid-chromatographic method was developed to quantify the release of tobramycin from polymeric drug delivery systems in vitro. Pre-column derivatization of tobramycin and kanamycin B sulphate (internal standard) was carried out with 2,4,6-trinitrobenzenesulphonic acid. The sample volume required was only 50 μl. The chromatographic separation was achieved on an octyl reversed-phase column with UV detection at 340 nm. This isocratic method was performed at ambient temperature and required only 8 min of chromatography time. The standard curves were linear over the concentration range 0.50-50.0 mg l-1. Inter-day and intra-day relative standard deviations ranged from 3.6 to 9.3% and from 1.6 to 6.8%, respectively. The assay method was used to determine the tobramycin content in different pharmaceutical formulations and to study the stability of the drug both in the solid-state and in solution.
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U2 - 10.1016/0731-7085(91)80152-Y
DO - 10.1016/0731-7085(91)80152-Y
M3 - Article
VL - 9
SP - 237
EP - 245
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
IS - 3
ER -