TY - JOUR
T1 - A liquid-chromatographic method for the determination of tobramycin
AU - Dash, A. K.
AU - Suryanarayanan, R.
N1 - Funding Information:
Acknowledgements - The authors thank Drs R.J. Sawchuk, M.A. Hedaya, R.P. Remmel (College of Pharmacy, University of Minnesota) and E. Kwong (Merck Frosst, Canada) for their valuable suggestions. Financial support was provided by the Graduate School, University of Minnesota and by the Merck Sharp and Dohme Research Laboratories.
PY - 1991
Y1 - 1991
N2 - A rapid and sensitive liquid-chromatographic method was developed to quantify the release of tobramycin from polymeric drug delivery systems in vitro. Pre-column derivatization of tobramycin and kanamycin B sulphate (internal standard) was carried out with 2,4,6-trinitrobenzenesulphonic acid. The sample volume required was only 50 μl. The chromatographic separation was achieved on an octyl reversed-phase column with UV detection at 340 nm. This isocratic method was performed at ambient temperature and required only 8 min of chromatography time. The standard curves were linear over the concentration range 0.50-50.0 mg l-1. Inter-day and intra-day relative standard deviations ranged from 3.6 to 9.3% and from 1.6 to 6.8%, respectively. The assay method was used to determine the tobramycin content in different pharmaceutical formulations and to study the stability of the drug both in the solid-state and in solution.
AB - A rapid and sensitive liquid-chromatographic method was developed to quantify the release of tobramycin from polymeric drug delivery systems in vitro. Pre-column derivatization of tobramycin and kanamycin B sulphate (internal standard) was carried out with 2,4,6-trinitrobenzenesulphonic acid. The sample volume required was only 50 μl. The chromatographic separation was achieved on an octyl reversed-phase column with UV detection at 340 nm. This isocratic method was performed at ambient temperature and required only 8 min of chromatography time. The standard curves were linear over the concentration range 0.50-50.0 mg l-1. Inter-day and intra-day relative standard deviations ranged from 3.6 to 9.3% and from 1.6 to 6.8%, respectively. The assay method was used to determine the tobramycin content in different pharmaceutical formulations and to study the stability of the drug both in the solid-state and in solution.
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U2 - 10.1016/0731-7085(91)80152-Y
DO - 10.1016/0731-7085(91)80152-Y
M3 - Article
C2 - 1873319
AN - SCOPUS:0025755887
VL - 9
SP - 237
EP - 245
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
IS - 3
ER -