A major human oral lysophosphatidic acid species, LPA 18

1, regulates novel genes in human gingival fibroblasts

D. Roselyn Cerutis, Michael Weston, Yazen Alnouti, Sai P. Bathena, Martha E. Nunn, Afolabi O. Ogunleye, Timothy P. McVaney, Karmel V. Headen, Takanari Miyamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The small bioactive lipid lysophosphatidic acid (LPA) plays critical roles in both normal physiology and inflammation in many systems. However, its actions are just beginning to be defined in oral biology and pathophysiology. Methods: Microarray analysis was used to test the hypothesis that human gingival fibroblasts (GFs) would show significant changes in wound-healing and inflammation-related gene transcripts in response to a major human salivary and gingival crevicular fluid LPA species, 18:1, and that they would express transcript for the major LPA-producing enzyme autotaxin. The microarray results were validated for three highly relevant upregulated inflammatory transcripts using quantitative reverse transcription-polymerase chain reaction (QRT-PCR). Liquid chromatography-tandem mass spectrometry was used to assay time-dependent LPA species production by GFs. Results: LPA 18:1 significantly regulated 20 GF novel and 27 known genes linked to the control of inflammation (P ≤0.01). QRT-PCR validation of interleukin (IL)-8, IL-11, and suppressor of cytokine signaling 2 (SOCS2) messenger RNAs confirmed statistically significant differences from control (P ≤0.05). Autotaxin transcript was present, and GFs were found to produce multiple LPA species in a time-dependent manner. Conclusions: The upregulation of transcripts for known GF proinflammatory ( IL-6, IL-8) and anti-inflammatory (IL-11) ILs, along with SOCS2, shows that LPA transiently regulates a complex set of GF genes critical to periodontal wound healing and inflammation. These results implicate LPA exerting actions on GFs that are compatible with functioning as a mediator in oral fibroblast biology and inflammatory responses. Therefore, LPA may potentially modulate/regulate periodontal inflammation.

Original languageEnglish
Pages (from-to)713-725
Number of pages13
JournalJournal of Periodontology
Volume86
Issue number5
DOIs
StatePublished - May 1 2015

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Fibroblasts
Genes
Inflammation
Interleukin-11
Interleukin-8
Wound Healing
Reverse Transcription
Gingival Crevicular Fluid
Cytokines
lysophosphatidic acid
Polymerase Chain Reaction
Microarray Analysis
Tandem Mass Spectrometry
Liquid Chromatography
Interleukin-6
Anti-Inflammatory Agents
Up-Regulation
Lipids
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

A major human oral lysophosphatidic acid species, LPA 18 : 1, regulates novel genes in human gingival fibroblasts. / Cerutis, D. Roselyn; Weston, Michael; Alnouti, Yazen; Bathena, Sai P.; Nunn, Martha E.; Ogunleye, Afolabi O.; McVaney, Timothy P.; Headen, Karmel V.; Miyamoto, Takanari.

In: Journal of Periodontology, Vol. 86, No. 5, 01.05.2015, p. 713-725.

Research output: Contribution to journalArticle

Cerutis, D. Roselyn ; Weston, Michael ; Alnouti, Yazen ; Bathena, Sai P. ; Nunn, Martha E. ; Ogunleye, Afolabi O. ; McVaney, Timothy P. ; Headen, Karmel V. ; Miyamoto, Takanari. / A major human oral lysophosphatidic acid species, LPA 18 : 1, regulates novel genes in human gingival fibroblasts. In: Journal of Periodontology. 2015 ; Vol. 86, No. 5. pp. 713-725.
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abstract = "Background: The small bioactive lipid lysophosphatidic acid (LPA) plays critical roles in both normal physiology and inflammation in many systems. However, its actions are just beginning to be defined in oral biology and pathophysiology. Methods: Microarray analysis was used to test the hypothesis that human gingival fibroblasts (GFs) would show significant changes in wound-healing and inflammation-related gene transcripts in response to a major human salivary and gingival crevicular fluid LPA species, 18:1, and that they would express transcript for the major LPA-producing enzyme autotaxin. The microarray results were validated for three highly relevant upregulated inflammatory transcripts using quantitative reverse transcription-polymerase chain reaction (QRT-PCR). Liquid chromatography-tandem mass spectrometry was used to assay time-dependent LPA species production by GFs. Results: LPA 18:1 significantly regulated 20 GF novel and 27 known genes linked to the control of inflammation (P ≤0.01). QRT-PCR validation of interleukin (IL)-8, IL-11, and suppressor of cytokine signaling 2 (SOCS2) messenger RNAs confirmed statistically significant differences from control (P ≤0.05). Autotaxin transcript was present, and GFs were found to produce multiple LPA species in a time-dependent manner. Conclusions: The upregulation of transcripts for known GF proinflammatory ( IL-6, IL-8) and anti-inflammatory (IL-11) ILs, along with SOCS2, shows that LPA transiently regulates a complex set of GF genes critical to periodontal wound healing and inflammation. These results implicate LPA exerting actions on GFs that are compatible with functioning as a mediator in oral fibroblast biology and inflammatory responses. Therefore, LPA may potentially modulate/regulate periodontal inflammation.",
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T1 - A major human oral lysophosphatidic acid species, LPA 18

T2 - 1, regulates novel genes in human gingival fibroblasts

AU - Cerutis, D. Roselyn

AU - Weston, Michael

AU - Alnouti, Yazen

AU - Bathena, Sai P.

AU - Nunn, Martha E.

AU - Ogunleye, Afolabi O.

AU - McVaney, Timothy P.

AU - Headen, Karmel V.

AU - Miyamoto, Takanari

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Background: The small bioactive lipid lysophosphatidic acid (LPA) plays critical roles in both normal physiology and inflammation in many systems. However, its actions are just beginning to be defined in oral biology and pathophysiology. Methods: Microarray analysis was used to test the hypothesis that human gingival fibroblasts (GFs) would show significant changes in wound-healing and inflammation-related gene transcripts in response to a major human salivary and gingival crevicular fluid LPA species, 18:1, and that they would express transcript for the major LPA-producing enzyme autotaxin. The microarray results were validated for three highly relevant upregulated inflammatory transcripts using quantitative reverse transcription-polymerase chain reaction (QRT-PCR). Liquid chromatography-tandem mass spectrometry was used to assay time-dependent LPA species production by GFs. Results: LPA 18:1 significantly regulated 20 GF novel and 27 known genes linked to the control of inflammation (P ≤0.01). QRT-PCR validation of interleukin (IL)-8, IL-11, and suppressor of cytokine signaling 2 (SOCS2) messenger RNAs confirmed statistically significant differences from control (P ≤0.05). Autotaxin transcript was present, and GFs were found to produce multiple LPA species in a time-dependent manner. Conclusions: The upregulation of transcripts for known GF proinflammatory ( IL-6, IL-8) and anti-inflammatory (IL-11) ILs, along with SOCS2, shows that LPA transiently regulates a complex set of GF genes critical to periodontal wound healing and inflammation. These results implicate LPA exerting actions on GFs that are compatible with functioning as a mediator in oral fibroblast biology and inflammatory responses. Therefore, LPA may potentially modulate/regulate periodontal inflammation.

AB - Background: The small bioactive lipid lysophosphatidic acid (LPA) plays critical roles in both normal physiology and inflammation in many systems. However, its actions are just beginning to be defined in oral biology and pathophysiology. Methods: Microarray analysis was used to test the hypothesis that human gingival fibroblasts (GFs) would show significant changes in wound-healing and inflammation-related gene transcripts in response to a major human salivary and gingival crevicular fluid LPA species, 18:1, and that they would express transcript for the major LPA-producing enzyme autotaxin. The microarray results were validated for three highly relevant upregulated inflammatory transcripts using quantitative reverse transcription-polymerase chain reaction (QRT-PCR). Liquid chromatography-tandem mass spectrometry was used to assay time-dependent LPA species production by GFs. Results: LPA 18:1 significantly regulated 20 GF novel and 27 known genes linked to the control of inflammation (P ≤0.01). QRT-PCR validation of interleukin (IL)-8, IL-11, and suppressor of cytokine signaling 2 (SOCS2) messenger RNAs confirmed statistically significant differences from control (P ≤0.05). Autotaxin transcript was present, and GFs were found to produce multiple LPA species in a time-dependent manner. Conclusions: The upregulation of transcripts for known GF proinflammatory ( IL-6, IL-8) and anti-inflammatory (IL-11) ILs, along with SOCS2, shows that LPA transiently regulates a complex set of GF genes critical to periodontal wound healing and inflammation. These results implicate LPA exerting actions on GFs that are compatible with functioning as a mediator in oral fibroblast biology and inflammatory responses. Therefore, LPA may potentially modulate/regulate periodontal inflammation.

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