TY - JOUR
T1 - A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins
AU - Beisel, Kirk W.
AU - Srinivasappa, Javaraiah
AU - Olsen, Margaret R.
AU - Stiff, Anne C.
AU - Essani, Karim
AU - Prabhakar, Bellur S.
PY - 1990/2
Y1 - 1990/2
N2 - A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.
AB - A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.
UR - http://www.scopus.com/inward/record.url?scp=0025158339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025158339&partnerID=8YFLogxK
U2 - 10.1016/0882-4010(90)90079-6
DO - 10.1016/0882-4010(90)90079-6
M3 - Article
C2 - 2161486
AN - SCOPUS:0025158339
VL - 8
SP - 151
EP - 156
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
SN - 0882-4010
IS - 2
ER -