A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins

Kirk Beisel, Javaraiah Srinivasappa, Margaret R. Olsen, Anne C. Stiff, Karim Essani, Bellur S. Prabhakar

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalMicrobial Pathogenesis
Volume8
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

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Cardiac Myosins
Contractile Proteins
Muscle Proteins
Enterovirus
Neutralizing Antibodies
Monoclonal Antibodies
Actins
Western Blotting
Skeletal Muscle Myosins
Molecular Mimicry
Viral Structural Proteins
Myosin Heavy Chains
Cross Reactions
Myocarditis
Capsid Proteins
Autoimmunity
Staining and Labeling
Peptides
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins. / Beisel, Kirk; Srinivasappa, Javaraiah; Olsen, Margaret R.; Stiff, Anne C.; Essani, Karim; Prabhakar, Bellur S.

In: Microbial Pathogenesis, Vol. 8, No. 2, 1990, p. 151-156.

Research output: Contribution to journalArticle

Beisel, Kirk ; Srinivasappa, Javaraiah ; Olsen, Margaret R. ; Stiff, Anne C. ; Essani, Karim ; Prabhakar, Bellur S. / A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins. In: Microbial Pathogenesis. 1990 ; Vol. 8, No. 2. pp. 151-156.
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