A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins

Kirk W. Beisel; Javaraiah Srinivasappa; Margaret R. Olsen; Anne C. Stiff; Karim Essani; Bellur S. Prabhakar

doi:10.1016/0882-4010(90)90079-6

A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins

Kirk W. Beisel, Javaraiah Srinivasappa, Margaret R. Olsen, Anne C. Stiff, Karim Essani, Bellur S. Prabhakar

Research output: Contribution to journal › Article

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Abstract

A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.

Original language	English (US)
Pages (from-to)	151-156
Number of pages	6
Journal	Microbial Pathogenesis
Volume	8
Issue number	2
DOIs	https://doi.org/10.1016/0882-4010(90)90079-6
State	Published - Feb 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Infectious Diseases

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Beisel, K. W., Srinivasappa, J., Olsen, M. R., Stiff, A. C., Essani, K., & Prabhakar, B. S. (1990). A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins. Microbial Pathogenesis, 8(2), 151-156. https://doi.org/10.1016/0882-4010(90)90079-6

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title = "A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins",

abstract = "A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.",

author = "Beisel, {Kirk W.} and Javaraiah Srinivasappa and Olsen, {Margaret R.} and Stiff, {Anne C.} and Karim Essani and Prabhakar, {Bellur S.}",

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T1 - A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins

AU - Beisel, Kirk W.

AU - Srinivasappa, Javaraiah

AU - Olsen, Margaret R.

AU - Stiff, Anne C.

AU - Essani, Karim

AU - Prabhakar, Bellur S.

PY - 1990/2

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N2 - A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.

AB - A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.

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