A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins

Kirk W. Beisel, Javaraiah Srinivasappa, Margaret R. Olsen, Anne C. Stiff, Karim Essani, Bellur S. Prabhakar

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalMicrobial Pathogenesis
Volume8
Issue number2
DOIs
StatePublished - Feb 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

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    Beisel, K. W., Srinivasappa, J., Olsen, M. R., Stiff, A. C., Essani, K., & Prabhakar, B. S. (1990). A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins. Microbial Pathogenesis, 8(2), 151-156. https://doi.org/10.1016/0882-4010(90)90079-6