Abstract
Creatinine (CRN) is a vertebrate metabolic waste product normally found in blood and urine. Previous work demonstrated that the hydrochloride salt of creatinine (CRN-HCl) acted as a potent inhibitor of bacterial replication. Creatinine hydrochloride does not inhibit the growth of yeasts or molds (i.e. fungi), making it a potentially useful addition to growth media to facilitate isolation of environmental or clinically relevant fungal species. Sabouraud dextrose agar is the current medium of choice for detection and isolation of fungi although it does not offer optimal nutritional requirements for some fungi and can permit growth of bacteria which may subsequently inhibit fungal growth and/or obscure fungal isolation. We show that CRN-HCl effectively suppresses bacterial growth in either liquid or solid agar media while allowing outgrowth of slower growing fungi using either experimentally prepared samples or environmental samples.
| Original language | English |
|---|---|
| Pages (from-to) | 155-161 |
| Number of pages | 7 |
| Journal | Journal of Microbiological Methods |
| Volume | 105 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
- Microbiology (medical)
- Medicine(all)
Cite this
A novel, broadly applicable approach to isolation of fungi in diverse growth media. / Smithee, Shane; Tracy, Steven; Drescher, Kristen M.; Pitz, Lisa A.; McDonald, Thomas.
In: Journal of Microbiological Methods, Vol. 105, 2014, p. 155-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel, broadly applicable approach to isolation of fungi in diverse growth media
AU - Smithee, Shane
AU - Tracy, Steven
AU - Drescher, Kristen M.
AU - Pitz, Lisa A.
AU - McDonald, Thomas
PY - 2014
Y1 - 2014
N2 - Creatinine (CRN) is a vertebrate metabolic waste product normally found in blood and urine. Previous work demonstrated that the hydrochloride salt of creatinine (CRN-HCl) acted as a potent inhibitor of bacterial replication. Creatinine hydrochloride does not inhibit the growth of yeasts or molds (i.e. fungi), making it a potentially useful addition to growth media to facilitate isolation of environmental or clinically relevant fungal species. Sabouraud dextrose agar is the current medium of choice for detection and isolation of fungi although it does not offer optimal nutritional requirements for some fungi and can permit growth of bacteria which may subsequently inhibit fungal growth and/or obscure fungal isolation. We show that CRN-HCl effectively suppresses bacterial growth in either liquid or solid agar media while allowing outgrowth of slower growing fungi using either experimentally prepared samples or environmental samples.
AB - Creatinine (CRN) is a vertebrate metabolic waste product normally found in blood and urine. Previous work demonstrated that the hydrochloride salt of creatinine (CRN-HCl) acted as a potent inhibitor of bacterial replication. Creatinine hydrochloride does not inhibit the growth of yeasts or molds (i.e. fungi), making it a potentially useful addition to growth media to facilitate isolation of environmental or clinically relevant fungal species. Sabouraud dextrose agar is the current medium of choice for detection and isolation of fungi although it does not offer optimal nutritional requirements for some fungi and can permit growth of bacteria which may subsequently inhibit fungal growth and/or obscure fungal isolation. We show that CRN-HCl effectively suppresses bacterial growth in either liquid or solid agar media while allowing outgrowth of slower growing fungi using either experimentally prepared samples or environmental samples.
UR - http://www.scopus.com/inward/record.url?scp=84907301521&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907301521&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2014.07.023
DO - 10.1016/j.mimet.2014.07.023
M3 - Article
VL - 105
SP - 155
EP - 161
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
ER -