A phylogeny of Caenorhabditis reveals frequent loss of introns during nematode evolution

Soochin Cho, Suk Won Jin, Adam Cohen, Ronald E. Ellis

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Since introns were discovered 26 years ago, people have wondered how changes in intron/exon structure occur, and what role these changes play in evolution. To answer these questions, we have begun studying gene structure in nematodes related to Caenorhabditis elegans. As a first step, we cloned a set of five genes from six different Caenorhabditis species, and used their amino acid sequences to construct the first detailed phylogeny of this genus. Our data indicate that nematode introns are lost at a very high rate during evolution, almost 400-fold higher than in mammals. These losses do not occur randomly, but instead, favor some introns and do not affect others. In contrast, intron gains are far less common than losses in these genes. On the basis of the sequences at each intron site, we suggest that several distinct mechanisms can cause introns to be lost. The small size of C. elegans introns should increase the rate at which each of these types of loss can occur, and might account for the dramatic difference in loss rate between nematodes and mammals.

Original languageEnglish
Pages (from-to)1207-1220
Number of pages14
JournalGenome Research
Volume14
Issue number7
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Caenorhabditis
Phylogeny
Introns
Caenorhabditis elegans
Mammals
Genes
Amino Acid Sequence
Exons

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

A phylogeny of Caenorhabditis reveals frequent loss of introns during nematode evolution. / Cho, Soochin; Jin, Suk Won; Cohen, Adam; Ellis, Ronald E.

In: Genome Research, Vol. 14, No. 7, 07.2004, p. 1207-1220.

Research output: Contribution to journalArticle

Cho, Soochin ; Jin, Suk Won ; Cohen, Adam ; Ellis, Ronald E. / A phylogeny of Caenorhabditis reveals frequent loss of introns during nematode evolution. In: Genome Research. 2004 ; Vol. 14, No. 7. pp. 1207-1220.
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