A pilot study to evaluate a tissue-engineered bilayered cell therapy as an alternative to tissue from the palate

Michael K. McGuire, E. Todd Scheyer, Martha E. Nunn, Philip T. Lavin

Research output: Contribution to journalArticle

65 Scopus citations


Background: This study evaluated the safety and effectiveness of a tissue-engineered skin product composed of viable neonatal keratinocytes and fibroblasts and compared it to a free gingival graft (FGG) in a procedure to enhance keratinized tissue (KT) and wound healing around teeth that do not require root coverage. Methods: Twenty-five subjects were enrolled who had at least two non-adjacent teeth in contralateral quadrants exhibiting an insufficient zone of attached gingiva requiring soft tissue grafting where root coverage was not desired. One tooth was randomized to receive an FGG, and the other was randomized to receive bilayered cell therapy (BCT). The amount of KT was measured at baseline and 3 and 6 months, and the texture and color of the grafted tissue were compared to the surrounding tissue at months 1, 3, and 6. A questionnaire was used to determine subject preference at 6 months. Biopsies and persistence studies were performed on a subset of the subjects. Results: The FGG generated statistically significantly (P

Original languageEnglish
Pages (from-to)1847-1856
Number of pages10
JournalJournal of Periodontology
Issue number10
StatePublished - Oct 2008
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this