A proper transmembrane Ca2+ gradient is essential for the higher enzymatic activity of adenylate cyclase

F. Y. Yang, Y. P. Tu

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Abstract

Adenylate cyclase from bovine brain cortex was reconstituted into liposomes with (1000 fold) or without transmembrane Ca2+ gradient. The highest enzyme activity (the active center of enzyme exposing outside) was observed in the vesicles with lower Ca2+ concentration outside (≈10-6 M, similar to physiological condition). If the transmembrane Ca2+ gradient was in the inverse direction (i.e. higher Ca2+ concentration outside, 1 mM), a lowest enzyme activity would appear. Such a difference could be diminished following addition of A23187. Obtained results showed that a proper transmembrane Ca2+ gradient is essential for the optimal fluidity of phospholipid bilayer, favouring the formation of suitable conformation of adenylate cyclase with higher enzyme activity.

Original languageEnglish (US)
Pages (from-to)366-371
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number2
DOIs
StatePublished - Mar 15 1991

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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