A simple HPLC method using a microbore column for the analysis of doxorubicin

Ping Zhao, Alekha K. Dash

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Doxorubicin is one of the most potent anti-tumor agents generally used in the treatment of bone cancer. A simple and sensitive HPLC method was developed and validated for the assay of doxorubicin. The method used a C 18 Luna microbore column (50x1 mm) with a fluorescent detector (505 nm Ex. and 550 nm Em.). The mobile phase consisted of water-acetonitrile-acetic acid (80:19:1, v/v/v, pH 3.0) and the flow rate was 0.1 ml min -1. Daunomycin was used as the internal standard. This isocratic system required a 10-min run-time, giving a detection limit of 0.02 ng (0.035 pmol per injection). Standard curves were linear over the concentration range of 0.01-0.1 μg ml -1. Relative standard deviations (R.S.D.) for the within-day, day-to-day precision, and the accuracy measurement for the assay were less than 4.0, 3.2, and 4.1%, respectively. This HPLC method was used to study the in vitro release characteristics of doxorubicin from implantable drug delivery system. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)543-548
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume20
Issue number3
DOIs
StatePublished - Jul 1999

Fingerprint

Doxorubicin
High Pressure Liquid Chromatography
Assays
Implantable Infusion Pumps
Bone Neoplasms
Daunorubicin
Acetic Acid
Limit of Detection
Tumors
Bone
Flow rate
Detectors
Injections
Water
Neoplasms
Therapeutics
acetonitrile
In Vitro Techniques
Drug Delivery Systems

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

A simple HPLC method using a microbore column for the analysis of doxorubicin. / Zhao, Ping; Dash, Alekha K.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 20, No. 3, 07.1999, p. 543-548.

Research output: Contribution to journalArticle

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