A simple HPLC method using a microbore column for the analysis of doxorubicin

Ping Zhao, Alekha K. Dash

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Doxorubicin is one of the most potent anti-tumor agents generally used in the treatment of bone cancer. A simple and sensitive HPLC method was developed and validated for the assay of doxorubicin. The method used a C 18 Luna microbore column (50x1 mm) with a fluorescent detector (505 nm Ex. and 550 nm Em.). The mobile phase consisted of water-acetonitrile-acetic acid (80:19:1, v/v/v, pH 3.0) and the flow rate was 0.1 ml min -1. Daunomycin was used as the internal standard. This isocratic system required a 10-min run-time, giving a detection limit of 0.02 ng (0.035 pmol per injection). Standard curves were linear over the concentration range of 0.01-0.1 μg ml -1. Relative standard deviations (R.S.D.) for the within-day, day-to-day precision, and the accuracy measurement for the assay were less than 4.0, 3.2, and 4.1%, respectively. This HPLC method was used to study the in vitro release characteristics of doxorubicin from implantable drug delivery system. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)543-548
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number3
StatePublished - Jul 1999

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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