A species-specific population of tyrosine hydroxylase-immunoreactive neurons in the medial amygdaloid nucleus of the Syrian hamster

Stephen E. Asmus, Anthony Kincaid, Sarah Winans Newman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The medial amygdaloid nucleus (Me) is part of a neural pathway that regulates sexual behavior in the male Syrian hamster. To characterize the neurochemical content of neurons in this nucleus, brains from colchicine-treated adult male and female hamsters were immunocytochemically labeled using antibodies that recognize the catecholamine-synthesizing enzymes, tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT), as well as dopamine: A large population of TH-immunoreactive (TH-IR) neurons was observed throughout Me of male and female hamsters, primarily concentrated in the midrostral and caudal portions of the nucleus. The somata were generally small to medium in size and bipolar. Brains from animals that did not receive colchicine contained a limited number of TH-IR neurons in Me as reported previously. The DBH and PNMT antisera did not label any cells in Me of colchicine-treated animals, and the dopamine antiserum labeled neurons in the same location as the caudal group of TH-IR cells. Therefore, these caudal TH-IR neurons are interpreted to be dopaminergic. The rostral group of TH-IR neurons, on the other hand, may be producing only the immediate precursor of dopamine, l-3,4-dihydroxyphenylalanine (L-DOPA). The TH-synthesizing neurons in Me of the Syrian hamster appear to be a species-specific group of cells located outside of the previously described catecholaminergic cell groups.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
JournalBrain Research
Volume575
Issue number2
DOIs
StatePublished - Mar 20 1992
Externally publishedYes

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Mesocricetus
Tyrosine 3-Monooxygenase
Neurons
Dopamine
Colchicine
Population
Phenylethanolamine N-Methyltransferase
Cricetinae
Immune Sera
Neural Pathways
Dihydroxyphenylalanine
Brain
Carisoprodol
Mixed Function Oxygenases
Corticomedial Nuclear Complex
Sexual Behavior
Catecholamines
Antibodies
Enzymes

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

A species-specific population of tyrosine hydroxylase-immunoreactive neurons in the medial amygdaloid nucleus of the Syrian hamster. / Asmus, Stephen E.; Kincaid, Anthony; Newman, Sarah Winans.

In: Brain Research, Vol. 575, No. 2, 20.03.1992, p. 199-207.

Research output: Contribution to journalArticle

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