A specific monovalent metal ion integral to the AA platform of the RNA tetraloop receptor

Soumitra Basu, Robert P. Rambo, Juliane K. Strauss-Soukup, Jamie H. Cate, Adrian R. Ferré-D'Amaré, Scott A. Strobel, Jennifer A. Doudna

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Metal ions are essential for the folding and activity of large catalytic RNAs. While divalent metal ions have been directly implicated in RNA tertiary structure formation, the role of monovalent ions has been largely unexplored. Here we report the first specific monovalent metal ion binding site within a catalytic RNA. As seen crystallographically, a potassium ion is coordinated immediately below AA platforms of the Tetrahymena ribozyme P4-P6 domain, including that within the tetraloop receptor. Interference and kinetic experiments demonstrate that potassium ion binding within the tetraloop receptor stabilizes the folding of the P4-P6 domain and enhances the activity of the Azoarcus group I intron. Since a monovalent ion binding site is integral to the tetraloop receptor, a tertiary structural motif that occurs frequently in RNA, monovalent metal ions are likely to participate in the folding and activity of a wide diversity of RNAs.

Original languageEnglish
Pages (from-to)986-992
Number of pages7
JournalNature Structural Biology
Volume5
Issue number11
DOIs
StatePublished - Nov 1998
Externally publishedYes

Fingerprint

Catalytic RNA
Metal ions
Metals
RNA
Ions
Potassium
Binding Sites
Introns
Azoarcus
Tetrahymena
Kinetics
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Basu, S., Rambo, R. P., Strauss-Soukup, J. K., Cate, J. H., Ferré-D'Amaré, A. R., Strobel, S. A., & Doudna, J. A. (1998). A specific monovalent metal ion integral to the AA platform of the RNA tetraloop receptor. Nature Structural Biology, 5(11), 986-992. https://doi.org/10.1038/2960

A specific monovalent metal ion integral to the AA platform of the RNA tetraloop receptor. / Basu, Soumitra; Rambo, Robert P.; Strauss-Soukup, Juliane K.; Cate, Jamie H.; Ferré-D'Amaré, Adrian R.; Strobel, Scott A.; Doudna, Jennifer A.

In: Nature Structural Biology, Vol. 5, No. 11, 11.1998, p. 986-992.

Research output: Contribution to journalArticle

Basu, S, Rambo, RP, Strauss-Soukup, JK, Cate, JH, Ferré-D'Amaré, AR, Strobel, SA & Doudna, JA 1998, 'A specific monovalent metal ion integral to the AA platform of the RNA tetraloop receptor', Nature Structural Biology, vol. 5, no. 11, pp. 986-992. https://doi.org/10.1038/2960
Basu, Soumitra ; Rambo, Robert P. ; Strauss-Soukup, Juliane K. ; Cate, Jamie H. ; Ferré-D'Amaré, Adrian R. ; Strobel, Scott A. ; Doudna, Jennifer A. / A specific monovalent metal ion integral to the AA platform of the RNA tetraloop receptor. In: Nature Structural Biology. 1998 ; Vol. 5, No. 11. pp. 986-992.
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