Abnormal expression of molecular markers for bladder impermeability and differentiation in the urothelium of patients with interstitial cystitis

Gennady Slobodov, Michael Feloney, Christopher Gran, Kimberly D. Kyker, Robert E. Hurst, Daniel J. Culkin

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

Purpose: Despite a lack of consensus concerning the etiology of interstitial cystitis (IC) the loss of impermeability and other abnormalities of the urothelium are features of the disease. In this study the distribution of proteins involved with epithelial adhesion, cellular differentiation and bladder impermeability in urothelial biopsies were explored by the immunohistochemical assessment of E-cadherin, ZO-1, uroplakin and chondroitin sulfate. Materials and Methods: Biopsies obtained from 27 patients with IC and 7 controls were immediately fixed in formalin, immunohistochemically labeled for the described proteins and scored for protein expression, morphology and differentiation. Results: Only 3 IC samples appeared completely normal, while 24 of the 27 showed an abnormality in at least 1 marker and in 6 all 4 markers were abnormal. In patients vs controls findings were abnormal for uroplakin in 13 of 27 vs 1 of 7 (p = 0.085), for E-cadherin (over expressed) in 18 of 27 vs 0 of 7 (p = 0.0021), for ZO-1 in 11 of 27 vs 0 of 7 (p = 0.046) and for chondroitin sulfate in 15 of 27 vs 0 of 7 (p = 0.0054). The morphology/polarity score significantly correlated with ZO-1 (Pearson r = 0.3935, p = 0.0423) and chondroitin sulfate (Pearson r = 0.7079, p

Original languageEnglish (US)
Pages (from-to)1554-1558
Number of pages5
JournalJournal of Urology
Volume171
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Urology

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