Activation of α1-adrenoceptors increases [3H]inositol metabolism in rat vas deferens and caudal artery

Anthony W. Fox, Peter W. Abel, Kenneth P. Minneman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Rings of rat vas deferens and caudal artery were incubated with [3H]inositol in Krebs-Ringer bicarbonate buffer containing 10 mM lithium chloride, and the production of water-soluble [3H]inositol phosphates was monitored. Norepinephrine increased [3H]inositol phosphate accumulation 7-fold in rings from vas deferens and 3-fold in rings from caudal artery. Epinephrine, phenylephrine and methoxamine were as effective as norepinephrine, suggesting that these drugs are full agonists in causing this response. Prazosin, phentolamine and yohimbine completelu blocked the stimulation by norepinephrine in both tissues with potencies typical of blockade of α1-adrenoceptors. Despite a substantial receptor reserve for α1-adrenoceptor mediated contractile responses, clonidine, p-amino-clonidine, phenyl-propanolamine and ephedrine can only cause a partial contractile response in rat vas deferens. However, all of these partial agonists were either as effective or more effective in increasing [3H]inositol phosphate accumulation in rat vas deferens as they were in activating a contractile response. These data suggest that α1-adrenoceptors increase phosphatidylinositol turnover in rat vas deferens and caudal artery, and that there may be a receptor reserve for α1-adrenoceptor mediated increases in [3H]inositol phosphate accumulation in these smooth muscles.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalEuropean Journal of Pharmacology
Volume116
Issue number1-2
DOIs
StatePublished - Oct 8 1985
Externally publishedYes

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Vas Deferens
Inositol
Inositol Phosphates
Adrenergic Receptors
Arteries
Norepinephrine
Clonidine
Propanolamines
Methoxamine
Lithium Chloride
Ephedrine
Yohimbine
Prazosin
Phentolamine
Phenylephrine
Bicarbonates
Phosphatidylinositols
Epinephrine
Smooth Muscle
Buffers

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Activation of α1-adrenoceptors increases [3H]inositol metabolism in rat vas deferens and caudal artery. / Fox, Anthony W.; Abel, Peter W.; Minneman, Kenneth P.

In: European Journal of Pharmacology, Vol. 116, No. 1-2, 08.10.1985, p. 145-152.

Research output: Contribution to journalArticle

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