Adsorption and decontamination of α-synuclein from medically and environmentally-relevant surfaces

Hanh T.M. Phan, Jason C. Bartz, Jacob Ayers, Benoit I. Giasson, Mathias Schubert, Keith B. Rodenhausen, Negin Kananizadeh, Yusong Li, Shannon L. Bartelt-Hunt

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The assembly and accumulation of α-synuclein fibrils are implicated in the development of several neurodegenerative disorders including multiple system atrophy and Parkinson's disease. Pre-existing α-synuclein fibrils can recruit and convert soluble non-fibrillar α-synuclein to the fibrillar form similar to what is observed in prion diseases. This raises concerns regarding attachment of fibrillary α-synuclein to medical instruments and subsequent exposure of patients to α-synuclein similar to what has been observed in iatrogenic transmission of prions. Here, we evaluated adsorption and desorption of α-synuclein to two surfaces: stainless steel and a gold surface coated with a 11-Amino-1-undecanethiol hydrochloride self-assembled-monolayer (SAM) using in-situ combinatorial quartz crystal microbalance with dissipation and spectroscopic ellipsometry. α-Synuclein was found to attach to both surfaces, however, increased α-synuclein adsorption was observed onto the positively charged SAM surface compared to the stainless steel surface. Dynamic light scattering data showed that larger α-synuclein fibrils were preferentially attached to the stainless steel surface when compared with the distributions in the original α-synuclein solution and on the SAM surface. We determined that after attachment, introduction of a 1N NaOH solution could completely remove α-synuclein adsorbed on the stainless steel surface while α-synuclein was retained on the SAM surface. Our results indicate α-synuclein can bind to multiple surface types and that decontamination is surface-dependent.

Original languageEnglish (US)
Pages (from-to)98-107
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume166
DOIs
StatePublished - Jun 1 2018

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Synucleins
decontamination
Decontamination
Adsorption
adsorption
Self assembled monolayers
Stainless Steel
stainless steels
Stainless steel
Prions
attachment
Parkinson disease
atrophy
Quartz Crystal Microbalance Techniques
Spectroscopic ellipsometry
Quartz crystal microbalances
Multiple System Atrophy
hydrochlorides
Dynamic light scattering
Prion Diseases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Adsorption and decontamination of α-synuclein from medically and environmentally-relevant surfaces. / Phan, Hanh T.M.; Bartz, Jason C.; Ayers, Jacob; Giasson, Benoit I.; Schubert, Mathias; Rodenhausen, Keith B.; Kananizadeh, Negin; Li, Yusong; Bartelt-Hunt, Shannon L.

In: Colloids and Surfaces B: Biointerfaces, Vol. 166, 01.06.2018, p. 98-107.

Research output: Contribution to journalArticle

Phan, HTM, Bartz, JC, Ayers, J, Giasson, BI, Schubert, M, Rodenhausen, KB, Kananizadeh, N, Li, Y & Bartelt-Hunt, SL 2018, 'Adsorption and decontamination of α-synuclein from medically and environmentally-relevant surfaces' Colloids and Surfaces B: Biointerfaces, vol. 166, pp. 98-107. https://doi.org/10.1016/j.colsurfb.2018.03.011
Phan, Hanh T.M. ; Bartz, Jason C. ; Ayers, Jacob ; Giasson, Benoit I. ; Schubert, Mathias ; Rodenhausen, Keith B. ; Kananizadeh, Negin ; Li, Yusong ; Bartelt-Hunt, Shannon L. / Adsorption and decontamination of α-synuclein from medically and environmentally-relevant surfaces. In: Colloids and Surfaces B: Biointerfaces. 2018 ; Vol. 166. pp. 98-107.
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