Alterations in prefrontal cortical serotonin and antidepressant-like behavior in a novel C3H/HeJxDBA/2J recombinant inbred mouse strain

Caroline A. Browne, Gerard Clarke, Joachim Hanke, Timothy G. Dinan, Herbert Schwegler, Deniz M. Yilmazer-Hanke, John F. Cryan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present study, two genetically related inbred mouse strains selectively bred for high and low fear-sensitized acoustic startle reflex (FSS) were assessed in the forced swim test model of anti-depressant action and central monoamine concentrations in several brain regions were investigated. These mice were generated through backcrossing C3H/HeJ mice on DBA/2J mice, followed by inbreeding for several generations. The high-FSS and low-FSS strains are known to differ in their acquisition and extinction of fear following auditory fear conditioning. Significantly increased concentrations of 5-HT and its metabolite 5-HIAA were observed in the medial prefrontal cortex (mPFC) but not in the hypothalamus, striatum, hippocampus, amygdala, or midbrain of high-FSS mice compared to low-FSS mice. In addition the concentration of DOPAC, the major metabolite of dopamine was also significantly increased in the mPFC. Furthermore, the high-FSS mice displayed significantly higher levels of immobility in the forced swim test but not the tail suspension test in comparison to the low-FSS group. The mPFC is not only important in the regulation of fear extinction, but also a key region of interest in the study of depression and maintenance of depressive-like behaviors. These data implicate serotonergic modulation in the mPFC in the maintenance of antidepressant-like behavior in a highly fearful mouse strain.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalBehavioural Brain Research
Volume236
Issue number1
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

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Inbred Strains Mice
Antidepressive Agents
Serotonin
Prefrontal Cortex
Fear
Inbreeding
Startle Reflex
Maintenance
Hindlimb Suspension
3,4-Dihydroxyphenylacetic Acid
Inbred DBA Mouse
Hydroxyindoleacetic Acid
Inbred C3H Mouse
Mesencephalon
Amygdala
Hypothalamus
Hippocampus
Dopamine
Depression
Brain

All Science Journal Classification (ASJC) codes

  • Behavioral Neuroscience

Cite this

Alterations in prefrontal cortical serotonin and antidepressant-like behavior in a novel C3H/HeJxDBA/2J recombinant inbred mouse strain. / Browne, Caroline A.; Clarke, Gerard; Hanke, Joachim; Dinan, Timothy G.; Schwegler, Herbert; Yilmazer-Hanke, Deniz M.; Cryan, John F.

In: Behavioural Brain Research, Vol. 236, No. 1, 01.01.2013, p. 283-288.

Research output: Contribution to journalArticle

Browne, Caroline A. ; Clarke, Gerard ; Hanke, Joachim ; Dinan, Timothy G. ; Schwegler, Herbert ; Yilmazer-Hanke, Deniz M. ; Cryan, John F. / Alterations in prefrontal cortical serotonin and antidepressant-like behavior in a novel C3H/HeJxDBA/2J recombinant inbred mouse strain. In: Behavioural Brain Research. 2013 ; Vol. 236, No. 1. pp. 283-288.
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