Abstract
Transgenic mice harboring simian virus 40 large T antigen (Tag) gene fused to an erythroid-specific enhancer developed soft tissue sarcomas which expressed very high levels of T antigen. The Tag expression was not detectable in the animals' non-transformed tissues. While mice bearing several copies of the transgene developed tumors at an early age of 4-6 months, those with a single copy had a delayed onset of 10-16 months, and DNA analysis of their tumors showed amplification of the Tag transgene. Amplification of a Tag transgene has also been described previously in brain tumors. Our studies demonstrate that Tag transgene amplification is not restricted to a particular construct or a single tumor type. Therefore, this may be a general mechanism for Tag-mediated carcinogenesis, and our transgenic mouse system can be useful for elucidating the mechanisms that govern the amplification process of Tag sequences in vivo.
Original language | English (US) |
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Pages (from-to) | 2049-2051 |
Number of pages | 3 |
Journal | Carcinogenesis |
Volume | 15 |
Issue number | 9 |
DOIs | |
State | Published - Sep 1994 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Cancer Research