Amplification of a sv40 t antigen transgene is associated with sarcomagenesis in mice

T. Teitz, T. S.B. Yen, Y. W. Kan

Research output: Contribution to journalArticlepeer-review


Transgenic mice harboring simian virus 40 large T antigen (Tag) gene fused to an erythroid-specific enhancer developed soft tissue sarcomas which expressed very high levels of T antigen. The Tag expression was not detectable in the animals' non-transformed tissues. While mice bearing several copies of the transgene developed tumors at an early age of 4-6 months, those with a single copy had a delayed onset of 10-16 months, and DNA analysis of their tumors showed amplification of the Tag transgene. Amplification of a Tag transgene has also been described previously in brain tumors. Our studies demonstrate that Tag transgene amplification is not restricted to a particular construct or a single tumor type. Therefore, this may be a general mechanism for Tag-mediated carcinogenesis, and our transgenic mouse system can be useful for elucidating the mechanisms that govern the amplification process of Tag sequences in vivo.

Original languageEnglish (US)
Pages (from-to)2049-2051
Number of pages3
Issue number9
StatePublished - Sep 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cancer Research


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