An ancient conserved gene expressed in the human inner ear

Identification, expression analysis, and chromosomal mapping of human and mouse antiquitin (ATQ1)

Anne B. Skvorak, Nahid G. Robertson, Yin Yi, Stanislawa Weremowicz, Her Helen, Frederick R. Bieber, Kirk Beisel, Eric D. Lynch, David R. Beier, Cynthia C. Morton

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We constructed and screened a human fetal cochlear cDNA library to identify genes involved in hearing and deafness. From this library we isolated a cDNA corresponding to the highly conserved ancient gene antiquitin (ATQ1). The plant homolog of ATQ1 is thought to be involved in regulating turgor pressure, a function that also would be essential for cells of the mammalian cochlea. Northern blots of 13 human fetal tissues show antiquitin to be highly expressed in cochlea, ovary, eye, heart, and kidney. Using RT- PCR of rat cochlear hair cell-specific cDNA libraries, we detect antiquitin expression in outer hair cells, but not in inner or vestibular type 1 hair cells, suggesting that antiquitin is not expressed ubiquitously in the cochlea. Human ATQ1 was mapped to human chromosome region 5q31 using fluorescence in situ hybridization, and mouse ATQ1 was mapped to mouse chromosome 18 by single-strand conformation polymorphism mapping of interspecific backcross progeny DNAs. Four human antiquitin-like sequences, possibly pseudogenes, were also identified and mapped.

Original languageEnglish
Pages (from-to)191-199
Number of pages9
JournalGenomics
Volume46
Issue number2
DOIs
StatePublished - Dec 1 1997

Fingerprint

Cochlea
Inner Ear
Gene Library
Outer Auditory Hair Cells
Genes
Auditory Hair Cells
Vestibular Hair Cells
Chromosomes, Human, Pair 18
Pseudogenes
Human Chromosomes
Deafness
Fluorescence In Situ Hybridization
Northern Blotting
Hearing
Libraries
Ovary
Fetus
Complementary DNA
Kidney
Pressure

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

An ancient conserved gene expressed in the human inner ear : Identification, expression analysis, and chromosomal mapping of human and mouse antiquitin (ATQ1). / Skvorak, Anne B.; Robertson, Nahid G.; Yi, Yin; Weremowicz, Stanislawa; Helen, Her; Bieber, Frederick R.; Beisel, Kirk; Lynch, Eric D.; Beier, David R.; Morton, Cynthia C.

In: Genomics, Vol. 46, No. 2, 01.12.1997, p. 191-199.

Research output: Contribution to journalArticle

Skvorak, AB, Robertson, NG, Yi, Y, Weremowicz, S, Helen, H, Bieber, FR, Beisel, K, Lynch, ED, Beier, DR & Morton, CC 1997, 'An ancient conserved gene expressed in the human inner ear: Identification, expression analysis, and chromosomal mapping of human and mouse antiquitin (ATQ1)', Genomics, vol. 46, no. 2, pp. 191-199. https://doi.org/10.1006/geno.1997.5026
Skvorak, Anne B. ; Robertson, Nahid G. ; Yi, Yin ; Weremowicz, Stanislawa ; Helen, Her ; Bieber, Frederick R. ; Beisel, Kirk ; Lynch, Eric D. ; Beier, David R. ; Morton, Cynthia C. / An ancient conserved gene expressed in the human inner ear : Identification, expression analysis, and chromosomal mapping of human and mouse antiquitin (ATQ1). In: Genomics. 1997 ; Vol. 46, No. 2. pp. 191-199.
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