Abstract
The abilities of potential chemoprotectants to inhibit cytotoxicity of ricin have been determined in vitro, using the macrophage cell line J744A.1. Six compounds were tested: α-and β-galactopyranosylamine; N-bromoacetyl-α-d-galactopyranosylamine; N-bromoacetyl-β-D-galactopyranosylamine; N-bromoacetylglucopyranosylamine; and N-bromoacetylmannopyranosylamine. Of the six compounds which were tested, only N-bromoacetyl-α-D-galactopyranosylamine and N-bromoacetyl-β-D-galactopyranosylamine exhibited significant activity against ricin toxicity, as indicated by the release of lactate dehydrogenase (LDH) and aspartate aminotransferase (AST). The α-isomer provided greater protection against ricin toxicity and also exhibited less inherent cytotoxicity in the absence of ricin, as compared to the β-isomer. Neither the α-and β-galactopyranosylamines nor the glucose and mannose analogs were promising as potential chemoprotectants.
| Original language | English |
|---|---|
| Pages (from-to) | 1545-1554 |
| Number of pages | 10 |
| Journal | Toxicon |
| Volume | 30 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1992 |
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All Science Journal Classification (ASJC) codes
- Toxicology
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An assessment of potential chemoprotectant activity against ricin toxicity by mechanism based glycosidase inhibitors in macrophage J744A.1 cell cultures. / Hassoun, E. A.; Bagchi, D.; Roche, Victoria F.; Stohs, S. J.
In: Toxicon, Vol. 30, No. 12, 1992, p. 1545-1554.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An assessment of potential chemoprotectant activity against ricin toxicity by mechanism based glycosidase inhibitors in macrophage J744A.1 cell cultures
AU - Hassoun, E. A.
AU - Bagchi, D.
AU - Roche, Victoria F.
AU - Stohs, S. J.
PY - 1992
Y1 - 1992
N2 - The abilities of potential chemoprotectants to inhibit cytotoxicity of ricin have been determined in vitro, using the macrophage cell line J744A.1. Six compounds were tested: α-and β-galactopyranosylamine; N-bromoacetyl-α-d-galactopyranosylamine; N-bromoacetyl-β-D-galactopyranosylamine; N-bromoacetylglucopyranosylamine; and N-bromoacetylmannopyranosylamine. Of the six compounds which were tested, only N-bromoacetyl-α-D-galactopyranosylamine and N-bromoacetyl-β-D-galactopyranosylamine exhibited significant activity against ricin toxicity, as indicated by the release of lactate dehydrogenase (LDH) and aspartate aminotransferase (AST). The α-isomer provided greater protection against ricin toxicity and also exhibited less inherent cytotoxicity in the absence of ricin, as compared to the β-isomer. Neither the α-and β-galactopyranosylamines nor the glucose and mannose analogs were promising as potential chemoprotectants.
AB - The abilities of potential chemoprotectants to inhibit cytotoxicity of ricin have been determined in vitro, using the macrophage cell line J744A.1. Six compounds were tested: α-and β-galactopyranosylamine; N-bromoacetyl-α-d-galactopyranosylamine; N-bromoacetyl-β-D-galactopyranosylamine; N-bromoacetylglucopyranosylamine; and N-bromoacetylmannopyranosylamine. Of the six compounds which were tested, only N-bromoacetyl-α-D-galactopyranosylamine and N-bromoacetyl-β-D-galactopyranosylamine exhibited significant activity against ricin toxicity, as indicated by the release of lactate dehydrogenase (LDH) and aspartate aminotransferase (AST). The α-isomer provided greater protection against ricin toxicity and also exhibited less inherent cytotoxicity in the absence of ricin, as compared to the β-isomer. Neither the α-and β-galactopyranosylamines nor the glucose and mannose analogs were promising as potential chemoprotectants.
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U2 - 10.1016/0041-0101(92)90026-2
DO - 10.1016/0041-0101(92)90026-2
M3 - Article
VL - 30
SP - 1545
EP - 1554
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 12
ER -