An assessment of potential chemoprotectant activity against ricin toxicity by mechanism based glycosidase inhibitors in macrophage J744A.1 cell cultures

E. A. Hassoun, D. Bagchi, V. F. Roche, S. J. Stohs

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The abilities of potential chemoprotectants to inhibit cytotoxicity of ricin have been determined in vitro, using the macrophage cell line J744A.1. Six compounds were tested: α-and β-galactopyranosylamine; N-bromoacetyl-α-d-galactopyranosylamine; N-bromoacetyl-β-D-galactopyranosylamine; N-bromoacetylglucopyranosylamine; and N-bromoacetylmannopyranosylamine. Of the six compounds which were tested, only N-bromoacetyl-α-D-galactopyranosylamine and N-bromoacetyl-β-D-galactopyranosylamine exhibited significant activity against ricin toxicity, as indicated by the release of lactate dehydrogenase (LDH) and aspartate aminotransferase (AST). The α-isomer provided greater protection against ricin toxicity and also exhibited less inherent cytotoxicity in the absence of ricin, as compared to the β-isomer. Neither the α-and β-galactopyranosylamines nor the glucose and mannose analogs were promising as potential chemoprotectants.

Original languageEnglish (US)
Pages (from-to)1545-1554
Number of pages10
Issue number12
StatePublished - Dec 1992


All Science Journal Classification (ASJC) codes

  • Toxicology

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