An enzymatic treatment of soil-bound prions effectively inhibits replication

Samuel E. Saunders, Jason C. Bartz, Kurt C. Vercauteren, Shannon L. Bartelt-Hunt

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Chronic wasting disease (CWD) and scrapie can be transmitted through indirect environmental routes, possibly via soil, and a practical decontamination strategy for prion-contaminated soil is currently unavailable. In the laboratory, an enzymatic treatment under environmentally relevant conditions (22°C, pH 7.4) can degrade soil-bound PrPSc below the limits of Western blot detection. We developed and used a quantitative serial protein misfolding cyclic amplification (PMCA) protocol to characterize the amplification efficiency of treated soil samples relative to controls of known infectious titer. Our results suggest large (104- to >106-fold) decreases in soil-bound prion infectivity following enzyme treatment, demonstrating that a mild enzymatic treatment could effectively reduce the risk of prion disease transmission via soil or other environmental surfaces.

Original languageEnglish
Pages (from-to)4313-4317
Number of pages5
JournalApplied and Environmental Microbiology
Volume77
Issue number13
DOIs
StatePublished - Jul 2011

Fingerprint

Prions
prions
enzymatic treatment
Soil
soil
chronic wasting disease
amplification
PrPSc proteins
prion disease
scrapie
prion diseases
decontamination
disease transmission
Chronic Wasting Disease
polluted soils
infectivity
Scrapie
Western blotting
Prion Diseases
pathogenicity

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

An enzymatic treatment of soil-bound prions effectively inhibits replication. / Saunders, Samuel E.; Bartz, Jason C.; Vercauteren, Kurt C.; Bartelt-Hunt, Shannon L.

In: Applied and Environmental Microbiology, Vol. 77, No. 13, 07.2011, p. 4313-4317.

Research output: Contribution to journalArticle

Saunders, Samuel E. ; Bartz, Jason C. ; Vercauteren, Kurt C. ; Bartelt-Hunt, Shannon L. / An enzymatic treatment of soil-bound prions effectively inhibits replication. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 13. pp. 4313-4317.
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