TY - JOUR
T1 - An extracellular matrix response element in the promoter of the LpS1 genes of the sea urchin Lytechinus pictus
AU - Seid, Christopher A.
AU - Ramachandran, Ravi K.
AU - George, Jenny M.
AU - Govindarajan, Venkatesh
AU - González-Rimbau, Maria F.
AU - Flytzanis, Constantin N.
AU - Tomlinson, Craig R.
N1 - Funding Information:
We thank Drs Lisa Meffert, Yolanda Sanchez and Akif Uzman for critical comments and Drs B.P.Brandhorst, P.Cserjesi and W.H.Klein for the DNA constructs. This work was supported by Sigma Xi awards to R.K.R, J.G.M. and V.G., NIH grant GM53727 (C.N.F.) and American Heart Association grants 91G-441 and 93R-441 (C.R.T.).
PY - 1997/8/1
Y1 - 1997/8/1
N2 - The extracellular matrix (ECM) has been shown to play an important role in development and tissue-specific gene expression, yet the mechanism by which genes receive signals from the ECM is poorly understood. The aboral ectoderm-specific LpS1-α and -β genes of Lytechinus pictus, members of the Spec gene family, provide an excellent model system to study ECM-mediated gene regulation. Disruption of the ECM by preventing collagen deposition using the lathrytic agent β-aminopropionitrile (BAPN) inhibits LpS1 gene transcription. LpS1 transcription resumes after removal of BAPN and subsequent collagen reformation. Using a chloramphenicol acetyltransferase (CAT) reporter gene assay, we show that a 125 bp region of the LpS1-β promoter from -108 to +17 contains an ECM response element (ECM RE). Insertion of the 125 bp region into the promoter of the metallothionein gene of L. pictus, a gene unaffected by ECM disruption, caused the fused promoter to become ECM dependent. As with the endogenous LpS1 genes, CAT activity directed by the fused LpS1-β promoter resumed in embryos recovered from ECM disruption. A mutation in a cis-acting element called the proximal G-string, which lies in the 125 bp region, caused CAT activity levels in ECM-disrupted embryos to equal that of the wild-type LpS1-β promoter in ECM-intact embryos. These results suggest that the intact ECM normally transmits signals to inhibit repressor activity at the proximal G-string in aboral ectoderm cells. Consistent with these results were our findings which showed that in addition to expression in the aboral ectoderm, the proximal G-string mutation caused expression of the CAT gene in oral ectoderm cells. These studies suggested that the proximal G-string serves as a binding site for negative regulation of the LpS1 genes in oral ectoderm during development. We also examined trans-acting factors binding the proximal G-string following ECM disruption. Band shift gels revealed a predominant set of slower migrating nuclear proteins from ECM-disrupted embryos which bound the proximal G-string. This work suggested that ECM disruption initiates signaling that induces a repressor to bind the ECM RE and/or modifies ECM RE binding proteins, which in turn represses LpS1 gene activity.
AB - The extracellular matrix (ECM) has been shown to play an important role in development and tissue-specific gene expression, yet the mechanism by which genes receive signals from the ECM is poorly understood. The aboral ectoderm-specific LpS1-α and -β genes of Lytechinus pictus, members of the Spec gene family, provide an excellent model system to study ECM-mediated gene regulation. Disruption of the ECM by preventing collagen deposition using the lathrytic agent β-aminopropionitrile (BAPN) inhibits LpS1 gene transcription. LpS1 transcription resumes after removal of BAPN and subsequent collagen reformation. Using a chloramphenicol acetyltransferase (CAT) reporter gene assay, we show that a 125 bp region of the LpS1-β promoter from -108 to +17 contains an ECM response element (ECM RE). Insertion of the 125 bp region into the promoter of the metallothionein gene of L. pictus, a gene unaffected by ECM disruption, caused the fused promoter to become ECM dependent. As with the endogenous LpS1 genes, CAT activity directed by the fused LpS1-β promoter resumed in embryos recovered from ECM disruption. A mutation in a cis-acting element called the proximal G-string, which lies in the 125 bp region, caused CAT activity levels in ECM-disrupted embryos to equal that of the wild-type LpS1-β promoter in ECM-intact embryos. These results suggest that the intact ECM normally transmits signals to inhibit repressor activity at the proximal G-string in aboral ectoderm cells. Consistent with these results were our findings which showed that in addition to expression in the aboral ectoderm, the proximal G-string mutation caused expression of the CAT gene in oral ectoderm cells. These studies suggested that the proximal G-string serves as a binding site for negative regulation of the LpS1 genes in oral ectoderm during development. We also examined trans-acting factors binding the proximal G-string following ECM disruption. Band shift gels revealed a predominant set of slower migrating nuclear proteins from ECM-disrupted embryos which bound the proximal G-string. This work suggested that ECM disruption initiates signaling that induces a repressor to bind the ECM RE and/or modifies ECM RE binding proteins, which in turn represses LpS1 gene activity.
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U2 - 10.1093/nar/25.15.3175
DO - 10.1093/nar/25.15.3175
M3 - Article
C2 - 9224621
AN - SCOPUS:0030811267
VL - 25
SP - 3175
EP - 3182
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 15
ER -