An in vivo model of beta-adrenoceptor desensitization

B. T Y Chong, Devendra K. Agrawal, F. A. Romero, R. G. Townley

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chronic use of β2-agonists and increased production of inflammatory mediators during the late allergic reaction after antigen challenge results in the desensitization of β-adrenoceptors in the airways and the accompanying rise in nonspecific airway hyperresponsiveness. In this study, we established an in vivo model of β2-adrenoceptor desensitization in guinea pig airways by administration of IL-1β intratracheally or chronic albuterol by inhalation. In the establishment of β-adrenoceptor desensitization in response to both β-agonist or inflammatory mediator, baseline pulmonary function responses were established to methacholine and isoproterenol-induced relaxation of methacholine bronchoconstriction. This was followed by the administration of IL-1β (500 IU/d intratracheally for 2 days) or chronic albuterol (0.1 g/L by aerosol for 1 min three times a day for 10 days). After administration, the methacholine and isoproterenol-methacholine response was once again evaluated. Intratracheal administration of IL-1β or chronic administration of albuterol significantly decreased (p <0.05) the protective effect of isoproterenol on methacholine-induced bronchoconstriction, eliciting β-adrenoceptor desensitization in vivo. The in vivo model will be very useful in monitoring the effect of other potential drugs on β-adrenoceptor function in the airways. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalJournal of Pharmacological and Toxicological Methods
Volume40
Issue number2
DOIs
StatePublished - 1998

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Methacholine Chloride
Adrenergic Receptors
Albuterol
Interleukin-1
Isoproterenol
Bronchoconstriction
Aerosols
Inhalation
Hypersensitivity
Guinea Pigs
Antigens
Lung
Monitoring
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology

Cite this

An in vivo model of beta-adrenoceptor desensitization. / Chong, B. T Y; Agrawal, Devendra K.; Romero, F. A.; Townley, R. G.

In: Journal of Pharmacological and Toxicological Methods, Vol. 40, No. 2, 1998, p. 109-115.

Research output: Contribution to journalArticle

Chong, B. T Y ; Agrawal, Devendra K. ; Romero, F. A. ; Townley, R. G. / An in vivo model of beta-adrenoceptor desensitization. In: Journal of Pharmacological and Toxicological Methods. 1998 ; Vol. 40, No. 2. pp. 109-115.
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