Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction

Dennis G. Drescher, Glenn E. Green, Khalid M. Khan, Kavita Hajela, Kirk Beisel, Barbara J. Morley, Anil K. Gupta

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Thirteen GABAA receptor subunits were examined in the cochlea of the CBAJ mouse by PCR analysis. Total RNA was extracted from the auditory organs of 14-18-day-old animals, and mRNA was isolated using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with primers specific for nucleotide sequences representing GABAA subunits. PCR products corresponding to subunits α16, β13, and γ2 were amplified, whereas those representing γ1, γ3, and δ were not amplified above background. These results provide the most direct evidence to date that GABAA receptors composed of the detected subunits are expressed in the mammalian cochlea, lending new support to previous studies implicating GABA as a cochlear transmitter. The pronounced expression of α2 and α6 subunits suggests type II and "cerebellar-type" benzodiazepine pharmacology in the cochlea.

Original languageEnglish
Pages (from-to)1167-1170
Number of pages4
JournalJournal of Neurochemistry
Volume61
Issue number3
StatePublished - Sep 1993

Fingerprint

Polymerase chain reaction
Cochlea
GABA-A Receptors
Polymerase Chain Reaction
Transcription
Benzodiazepines
gamma-Aminobutyric Acid
Transmitters
Animals
Nucleotides
Complementary DNA
RNA
Messenger RNA
Reverse Transcription
Pharmacology
oligo(dT)-cellulose

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Drescher, D. G., Green, G. E., Khan, K. M., Hajela, K., Beisel, K., Morley, B. J., & Gupta, A. K. (1993). Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction. Journal of Neurochemistry, 61(3), 1167-1170.

Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction. / Drescher, Dennis G.; Green, Glenn E.; Khan, Khalid M.; Hajela, Kavita; Beisel, Kirk; Morley, Barbara J.; Gupta, Anil K.

In: Journal of Neurochemistry, Vol. 61, No. 3, 09.1993, p. 1167-1170.

Research output: Contribution to journalArticle

Drescher, DG, Green, GE, Khan, KM, Hajela, K, Beisel, K, Morley, BJ & Gupta, AK 1993, 'Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction', Journal of Neurochemistry, vol. 61, no. 3, pp. 1167-1170.
Drescher, Dennis G. ; Green, Glenn E. ; Khan, Khalid M. ; Hajela, Kavita ; Beisel, Kirk ; Morley, Barbara J. ; Gupta, Anil K. / Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction. In: Journal of Neurochemistry. 1993 ; Vol. 61, No. 3. pp. 1167-1170.
@article{d49fb30f669649108742d730e15a4ab3,
title = "Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction",
abstract = "Thirteen GABAA receptor subunits were examined in the cochlea of the CBAJ mouse by PCR analysis. Total RNA was extracted from the auditory organs of 14-18-day-old animals, and mRNA was isolated using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with primers specific for nucleotide sequences representing GABAA subunits. PCR products corresponding to subunits α1-α6, β1-β3, and γ2 were amplified, whereas those representing γ1, γ3, and δ were not amplified above background. These results provide the most direct evidence to date that GABAA receptors composed of the detected subunits are expressed in the mammalian cochlea, lending new support to previous studies implicating GABA as a cochlear transmitter. The pronounced expression of α2 and α6 subunits suggests type II and {"}cerebellar-type{"} benzodiazepine pharmacology in the cochlea.",
author = "Drescher, {Dennis G.} and Green, {Glenn E.} and Khan, {Khalid M.} and Kavita Hajela and Kirk Beisel and Morley, {Barbara J.} and Gupta, {Anil K.}",
year = "1993",
month = "9",
language = "English",
volume = "61",
pages = "1167--1170",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Analysis of γ-aminobutyric acidA receptor subunits in the mouse cochlea by means of the polymerase chain reaction

AU - Drescher, Dennis G.

AU - Green, Glenn E.

AU - Khan, Khalid M.

AU - Hajela, Kavita

AU - Beisel, Kirk

AU - Morley, Barbara J.

AU - Gupta, Anil K.

PY - 1993/9

Y1 - 1993/9

N2 - Thirteen GABAA receptor subunits were examined in the cochlea of the CBAJ mouse by PCR analysis. Total RNA was extracted from the auditory organs of 14-18-day-old animals, and mRNA was isolated using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with primers specific for nucleotide sequences representing GABAA subunits. PCR products corresponding to subunits α1-α6, β1-β3, and γ2 were amplified, whereas those representing γ1, γ3, and δ were not amplified above background. These results provide the most direct evidence to date that GABAA receptors composed of the detected subunits are expressed in the mammalian cochlea, lending new support to previous studies implicating GABA as a cochlear transmitter. The pronounced expression of α2 and α6 subunits suggests type II and "cerebellar-type" benzodiazepine pharmacology in the cochlea.

AB - Thirteen GABAA receptor subunits were examined in the cochlea of the CBAJ mouse by PCR analysis. Total RNA was extracted from the auditory organs of 14-18-day-old animals, and mRNA was isolated using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with primers specific for nucleotide sequences representing GABAA subunits. PCR products corresponding to subunits α1-α6, β1-β3, and γ2 were amplified, whereas those representing γ1, γ3, and δ were not amplified above background. These results provide the most direct evidence to date that GABAA receptors composed of the detected subunits are expressed in the mammalian cochlea, lending new support to previous studies implicating GABA as a cochlear transmitter. The pronounced expression of α2 and α6 subunits suggests type II and "cerebellar-type" benzodiazepine pharmacology in the cochlea.

UR - http://www.scopus.com/inward/record.url?scp=0027304610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027304610&partnerID=8YFLogxK

M3 - Article

C2 - 8395561

AN - SCOPUS:0027304610

VL - 61

SP - 1167

EP - 1170

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -