Analysis of γ‐Aminobutyric AcidA Receptor Subunits in the Mouse Cochlea by Means of the Polymerase Chain Reaction

Dennis G. Drescher, Glenn E. Green, Khalid M. Khan, Kavita Hajela, Kirk W. Beisel, Barbara J. Morley, Anil K. Gupta

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Abstract

Thirteen GABAA receptor subunits were examined in the cochlea of the CBAJ mouse by PCR analysis. Total RNA was extracted from the auditory organs of 14-18-day-old animals, and mRNA was isolated using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with primers specific for nucleotide sequences representing GABAA subunits. PCR products corresponding to subunits α16, β13, and γ2 were amplified, whereas those representing γ1, γ3, and δ were not amplified above background. These results provide the most direct evidence to date that GABAA receptors composed of the detected subunits are expressed in the mammalian cochlea, lending new support to previous studies implicating GABA as a cochlear transmitter. The pronounced expression of α2 and α6 subunits suggests type II and "cerebellar-type" benzodiazepine pharmacology in the cochlea.

Original languageEnglish (US)
Pages (from-to)1167-1170
Number of pages4
JournalJournal of Neurochemistry
Volume61
Issue number3
DOIs
StatePublished - Sep 1993

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Drescher, D. G., Green, G. E., Khan, K. M., Hajela, K., Beisel, K. W., Morley, B. J., & Gupta, A. K. (1993). Analysis of γ‐Aminobutyric AcidA Receptor Subunits in the Mouse Cochlea by Means of the Polymerase Chain Reaction. Journal of Neurochemistry, 61(3), 1167-1170. https://doi.org/10.1111/j.1471-4159.1993.tb03638.x