TY - JOUR
T1 - Analysis of CHEK2 gene for ovarian cancer susceptibility
AU - Baysal, Bora E.
AU - DeLoia, Julie A.
AU - Willett-Brozick, Joan E.
AU - Goodman, Marc T.
AU - Brady, Mark F.
AU - Modugno, Francesmary
AU - Lynch, Henry T.
AU - Conley, Yvette P.
AU - Watson, Patrice
AU - Gallion, Holly H.
N1 - Funding Information:
We thank Dr. Z. Miner and GOG Statistical and Data Center for help in data retrieval. This investigation was supported in part by Scaife Family Foundation, Anna L., and Irene V. Caplan Philanthropic Fund, The Anneliese Lermann Fund for Cancer Research, U.S. Public Health Service grants R01-CA-58598 and P30-CA-71789, K07-CA80668-01A1, R03CA92776, 1U01 CA86389, Department of Defense Grant DAMD17-001-0569, and contracts CN-55424 and PC-67001 from the National Cancer Institute (NCI) as well as NCI grants to the Gynecologic Oncology Group (GOG) Administrative Office and GOG Tissue Bank (CA27469) and to the GOG Statistical & Data Center (CA37517).
PY - 2004/10
Y1 - 2004/10
N2 - A deletion variant in the CHEK2 gene (del1100C) has been implicated as a low-penetrance risk factor for breast cancer. We sought to determine contribution of CHEK2 mutations to the etiology of ovarian cancer (OvCa). We used cases ascertained from the United States through Gynecologic Oncology Group (GOG) protocols 172, 182, and 144, the University of Hawaii Cancer Research Center, and Creighton University. Control women were recruited from Pittsburgh and Hawaii. Denaturing high-performance liquid chromatography, sequence analysis, and single nucleotide polymorphism genotyping by Pyrosequencing were employed to analyze the CHEK2 gene. Mutation screening of the CHEK2 gene in 48 cases who had a first-degree relative with OvCa uncovered only del1100C and A252G variants. Altogether, the del1100C variant was detected in none of 751 unselected cases, in 1 of 52 (1.9%) cases who had a first-degree relative with OvCa, and in 3 of 521 (0.6%) unselected controls. The frequencies of del1100C and A252G variants did not show statistically significant differences between the cases and the controls. These results suggest that variations in CHEK2 do not make a significant contribution to the pathogenesis of OvCa in the U.S. population.
AB - A deletion variant in the CHEK2 gene (del1100C) has been implicated as a low-penetrance risk factor for breast cancer. We sought to determine contribution of CHEK2 mutations to the etiology of ovarian cancer (OvCa). We used cases ascertained from the United States through Gynecologic Oncology Group (GOG) protocols 172, 182, and 144, the University of Hawaii Cancer Research Center, and Creighton University. Control women were recruited from Pittsburgh and Hawaii. Denaturing high-performance liquid chromatography, sequence analysis, and single nucleotide polymorphism genotyping by Pyrosequencing were employed to analyze the CHEK2 gene. Mutation screening of the CHEK2 gene in 48 cases who had a first-degree relative with OvCa uncovered only del1100C and A252G variants. Altogether, the del1100C variant was detected in none of 751 unselected cases, in 1 of 52 (1.9%) cases who had a first-degree relative with OvCa, and in 3 of 521 (0.6%) unselected controls. The frequencies of del1100C and A252G variants did not show statistically significant differences between the cases and the controls. These results suggest that variations in CHEK2 do not make a significant contribution to the pathogenesis of OvCa in the U.S. population.
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U2 - 10.1016/j.ygyno.2004.07.015
DO - 10.1016/j.ygyno.2004.07.015
M3 - Article
C2 - 15385111
AN - SCOPUS:4644273978
VL - 95
SP - 62
EP - 69
JO - Gynecologic Oncology
JF - Gynecologic Oncology
SN - 0090-8258
IS - 1
ER -