Aneuploid chromosomes are highly unstable during DNA transformation of candida albicans∇

Kelly Bouchonville, Anja Forche, Karen E S Tang, Anna Selmecki, Judith Berman

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Candida albicans strains tolerate aneuploidy, historically detected as karyotype alterations by pulsed-fleld gel electrophoresis and more recently revealed by array comparative genome hybridization, which provides a comprehensive and detailed description of gene copy number. Here, we first retrospectively analyzed 411 expression array experiments to predict the frequency of aneuploidy in different strains. As expected, significant levels of aneuploidy were seen in strains exposed to stress conditions, including UV light and/or sorbose treatment, as well as in strains that are resistant to antifungal drugs. More surprisingly, strains that underwent transformation with DNA displayed the highest frequency of chromosome copy number changes, with strains that were initially aneuploid exhibiting ∼3-fold more copy number changes than strains that were initially diploid. We then prospectively analyzed the effect of lithium acetate (LiOAc) transformation protocols on the stability of trisomie chromosomes. Consistent with the retrospective analysis, the proportion of karyotype changes was highly elevated in strains carrying aneuploid chromosomes. We then tested the hypothesis that stresses conferred by heat and/or LiOAc exposure promote chromosome number changes during DNA transformation procedures. Indeed, a short pulse of very high temperature caused frequent gains and losses of multiple chromosomes or chromosome segments. Furthermore, milder heat exposure over longer periods caused increased levels of loss of heterozygosity. Nonetheless, aneuploid chromosomes were also unstable when strains were transformed by electroporation, which does not include a heat shock step. Thus, aneuploid strains are particularly prone to undergo changes in chromosome number during the stresses of DNA transformation protocols.

Original languageEnglish
Pages (from-to)1554-1566
Number of pages13
JournalEukaryotic Cell
Volume8
Issue number10
DOIs
StatePublished - Oct 2009

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Aneuploidy
Candida albicans
Chromosomes
DNA
Hot Temperature
Karyotype
Sorbose
Chromosomal Instability
Comparative Genomic Hybridization
Electroporation
Gene Dosage
Loss of Heterozygosity
Ultraviolet Rays
Diploidy
Electrophoresis
Shock
Gels
Temperature
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Aneuploid chromosomes are highly unstable during DNA transformation of candida albicans∇. / Bouchonville, Kelly; Forche, Anja; Tang, Karen E S; Selmecki, Anna; Berman, Judith.

In: Eukaryotic Cell, Vol. 8, No. 10, 10.2009, p. 1554-1566.

Research output: Contribution to journalArticle

Bouchonville, Kelly ; Forche, Anja ; Tang, Karen E S ; Selmecki, Anna ; Berman, Judith. / Aneuploid chromosomes are highly unstable during DNA transformation of candida albicans∇. In: Eukaryotic Cell. 2009 ; Vol. 8, No. 10. pp. 1554-1566.
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