Aneuploidy and isochromosome formation in drug-resistant Candida albicans

Anna Selmecki, Anja Forche, Judith Berman

Research output: Contribution to journalArticle

365 Citations (Scopus)

Abstract

Resistance to the limited number of available antifungal drugs is a serious problem in the treatment of Candida albicans. We found that aneuploidy in general and a specific segmental aneuploidy, consisting of an isochromosome composed of the two left arms of chromosome 5, were associated with azole resistance. The isochromosome forms around a single centromere flanked by an inverted repeat and was found as an independent chromosome or fused at the telomere to a full-length homolog of chromosome 5. Increases and decreases in drug resistance were strongly associated with gain and loss of this isochromosome, which bears genes expressing the enzyme in the ergosterol pathway targeted by azole drugs, efflux pumps, and a transcription factor that positively regulates a subset of efflux pump genes.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalScience
Volume313
Issue number5785
DOIs
StatePublished - Jul 21 2006

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Isochromosomes
Aneuploidy
Candida albicans
Azoles
Chromosomes, Human, Pair 5
Pharmaceutical Preparations
Ergosterol
Centromere
Telomere
Drug Resistance
Genes
Transcription Factors
Chromosomes
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Aneuploidy and isochromosome formation in drug-resistant Candida albicans. / Selmecki, Anna; Forche, Anja; Berman, Judith.

In: Science, Vol. 313, No. 5785, 21.07.2006, p. 367-370.

Research output: Contribution to journalArticle

Selmecki, Anna ; Forche, Anja ; Berman, Judith. / Aneuploidy and isochromosome formation in drug-resistant Candida albicans. In: Science. 2006 ; Vol. 313, No. 5785. pp. 367-370.
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