Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica

Todd Isaacson, Ana Maria Soto, Shawichi Iwamuro, Floyd C. Knoop, J. Michael Conlon

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Japonicin-1 (FFPIGVFCKIFKTC) and japonicin-2 (FGLPMLSILPKALCILLKRKC), two peptides with differential growth-inhibitory activity against the Gram-negative bacterium, Escherichia coli and the Gram-positive bacterium Staphylococcus aureus, were isolated from an extract of the skin of the Japanese brown frog Rana japonica. Both peptides show little amino acid sequence similarity to previously characterized antimicrobial peptides isolated from the skins of Ranid frogs. Circular dichroism studies, however, demonstrate that japonicin-2 adopts an α-helical conformation in 50% trifluoroethanol in common with many other cationic antimicrobial peptides synthesized in amphibian skin. Peptides belonging to the brevinin-1, brevinin-2, and tigerinin families, previously identified in the skins of Asian Ranid frogs, were not detected but a temporin-related peptide (ILPLVGNLLNDLL.NH2; temporin-1Ja), that atypically bears no net positive charge, was isolated from the extract. The minimum inhibitory concentrations (MICs) of the peptides against E. coli were japonicin-1, 30 μM; japonicin-2, 12 μM; and temporin-1Ja > 100 μM. The MICs against S. aureus were japonicin-1, > 100 μM; japonicin-2, 20 μM; and temporin-1Ja, > 100 μM.

Original languageEnglish (US)
Pages (from-to)419-425
Number of pages7
JournalPeptides
Volume23
Issue number3
DOIs
StatePublished - Feb 23 2002

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Ranidae
Anura
Skin
Peptides
Microbial Sensitivity Tests
Staphylococcus aureus
Escherichia coli
Antimicrobial Cationic Peptides
Trifluoroethanol
Bacteria
Gram-Positive Bacteria
Amphibians
Circular Dichroism
Gram-Negative Bacteria
Amino Acid Sequence
Conformations
temporin
japonicin 2
Amino Acids
Growth

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica. / Isaacson, Todd; Soto, Ana Maria; Iwamuro, Shawichi; Knoop, Floyd C.; Conlon, J. Michael.

In: Peptides, Vol. 23, No. 3, 23.02.2002, p. 419-425.

Research output: Contribution to journalArticle

Isaacson, Todd ; Soto, Ana Maria ; Iwamuro, Shawichi ; Knoop, Floyd C. ; Conlon, J. Michael. / Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica. In: Peptides. 2002 ; Vol. 23, No. 3. pp. 419-425.
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