Anxiety-related behavior and densities of glutamate, GABAA, acetylcholine and serotonin receptors in the amygdala of seven inbred mouse strains

Deniz M. Yilmazer-Hanke, Thomas Roskoden, Karl Zilles, Herbert Schwegler

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The amygdala is a brain region involved in the regulation of anxiety-related behavior. The purpose of this study was to correlate anxiety-related behavior of inbred mouse strains (BA//c, BALB/cJ, C3H/HeJ, C57BL/6J, CPB-K, DBA/2J, NMRI) to receptor binding in the amygdala. Binding site densities of receptors (NMDA, AMPA, kainate, GABAA, serotonin, muscarinergic M1-M2) were measured with quantitative receptor autoradiography using tritiated ligands. Measurements of fear-sensitized acoustic startle response (ASR; induced by footshocks), elevated plus maze (EPM) behavior and receptor binding studies showed differences between the strains except for AMPA and muscarinergic M2 receptors. Factor analysis revealed a Startle Factor with positive loadings of the density of serotonin and kainate receptors, and the amplitudes of the baseline and fear-sensitized ASRs. A second Anxiety-related Factor only correlated with the fear-sensitized ASR and anxiety parameters on the EPM but not receptor densities. There were also two General Activity Factors defined by (negative) correlations with entries to closed arms of the EPM. Because the density of NMDA and muscarinergic M1 receptors also correlated negatively with the two factors, these receptors had a positive effect on general activity. In contrast, correlations of GABAA, serotonin, and kainate receptors had the opposite sign as compared to closed arm entries. It is concluded that hereditary variations in the amygdala, particularly in kainate and serotonin receptors, play a role for the baseline and fear-sensitized ASR, whereas the general activity is influenced by many neurotransmitter receptor systems.

Original languageEnglish
Pages (from-to)145-159
Number of pages15
JournalBehavioural Brain Research
Volume145
Issue number1-2
DOIs
StatePublished - Oct 17 2003
Externally publishedYes

Fingerprint

Inbred Strains Mice
Serotonin Receptors
Glutamate Receptors
Cholinergic Receptors
GABA-A Receptors
Amygdala
Kainic Acid Receptors
Fear
Anxiety
Startle Reflex
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Neurotransmitter Receptor
AMPA Receptors
Kainic Acid
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Autoradiography
Acoustics
Statistical Factor Analysis
Serotonin

All Science Journal Classification (ASJC) codes

  • Behavioral Neuroscience

Cite this

Anxiety-related behavior and densities of glutamate, GABAA, acetylcholine and serotonin receptors in the amygdala of seven inbred mouse strains. / Yilmazer-Hanke, Deniz M.; Roskoden, Thomas; Zilles, Karl; Schwegler, Herbert.

In: Behavioural Brain Research, Vol. 145, No. 1-2, 17.10.2003, p. 145-159.

Research output: Contribution to journalArticle

Yilmazer-Hanke, Deniz M. ; Roskoden, Thomas ; Zilles, Karl ; Schwegler, Herbert. / Anxiety-related behavior and densities of glutamate, GABAA, acetylcholine and serotonin receptors in the amygdala of seven inbred mouse strains. In: Behavioural Brain Research. 2003 ; Vol. 145, No. 1-2. pp. 145-159.
@article{623d0d5badd541e4b26eb7569b783b1e,
title = "Anxiety-related behavior and densities of glutamate, GABAA, acetylcholine and serotonin receptors in the amygdala of seven inbred mouse strains",
abstract = "The amygdala is a brain region involved in the regulation of anxiety-related behavior. The purpose of this study was to correlate anxiety-related behavior of inbred mouse strains (BA//c, BALB/cJ, C3H/HeJ, C57BL/6J, CPB-K, DBA/2J, NMRI) to receptor binding in the amygdala. Binding site densities of receptors (NMDA, AMPA, kainate, GABAA, serotonin, muscarinergic M1-M2) were measured with quantitative receptor autoradiography using tritiated ligands. Measurements of fear-sensitized acoustic startle response (ASR; induced by footshocks), elevated plus maze (EPM) behavior and receptor binding studies showed differences between the strains except for AMPA and muscarinergic M2 receptors. Factor analysis revealed a Startle Factor with positive loadings of the density of serotonin and kainate receptors, and the amplitudes of the baseline and fear-sensitized ASRs. A second Anxiety-related Factor only correlated with the fear-sensitized ASR and anxiety parameters on the EPM but not receptor densities. There were also two General Activity Factors defined by (negative) correlations with entries to closed arms of the EPM. Because the density of NMDA and muscarinergic M1 receptors also correlated negatively with the two factors, these receptors had a positive effect on general activity. In contrast, correlations of GABAA, serotonin, and kainate receptors had the opposite sign as compared to closed arm entries. It is concluded that hereditary variations in the amygdala, particularly in kainate and serotonin receptors, play a role for the baseline and fear-sensitized ASR, whereas the general activity is influenced by many neurotransmitter receptor systems.",
author = "Yilmazer-Hanke, {Deniz M.} and Thomas Roskoden and Karl Zilles and Herbert Schwegler",
year = "2003",
month = "10",
day = "17",
doi = "10.1016/S0166-4328(03)00107-4",
language = "English",
volume = "145",
pages = "145--159",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Anxiety-related behavior and densities of glutamate, GABAA, acetylcholine and serotonin receptors in the amygdala of seven inbred mouse strains

AU - Yilmazer-Hanke, Deniz M.

AU - Roskoden, Thomas

AU - Zilles, Karl

AU - Schwegler, Herbert

PY - 2003/10/17

Y1 - 2003/10/17

N2 - The amygdala is a brain region involved in the regulation of anxiety-related behavior. The purpose of this study was to correlate anxiety-related behavior of inbred mouse strains (BA//c, BALB/cJ, C3H/HeJ, C57BL/6J, CPB-K, DBA/2J, NMRI) to receptor binding in the amygdala. Binding site densities of receptors (NMDA, AMPA, kainate, GABAA, serotonin, muscarinergic M1-M2) were measured with quantitative receptor autoradiography using tritiated ligands. Measurements of fear-sensitized acoustic startle response (ASR; induced by footshocks), elevated plus maze (EPM) behavior and receptor binding studies showed differences between the strains except for AMPA and muscarinergic M2 receptors. Factor analysis revealed a Startle Factor with positive loadings of the density of serotonin and kainate receptors, and the amplitudes of the baseline and fear-sensitized ASRs. A second Anxiety-related Factor only correlated with the fear-sensitized ASR and anxiety parameters on the EPM but not receptor densities. There were also two General Activity Factors defined by (negative) correlations with entries to closed arms of the EPM. Because the density of NMDA and muscarinergic M1 receptors also correlated negatively with the two factors, these receptors had a positive effect on general activity. In contrast, correlations of GABAA, serotonin, and kainate receptors had the opposite sign as compared to closed arm entries. It is concluded that hereditary variations in the amygdala, particularly in kainate and serotonin receptors, play a role for the baseline and fear-sensitized ASR, whereas the general activity is influenced by many neurotransmitter receptor systems.

AB - The amygdala is a brain region involved in the regulation of anxiety-related behavior. The purpose of this study was to correlate anxiety-related behavior of inbred mouse strains (BA//c, BALB/cJ, C3H/HeJ, C57BL/6J, CPB-K, DBA/2J, NMRI) to receptor binding in the amygdala. Binding site densities of receptors (NMDA, AMPA, kainate, GABAA, serotonin, muscarinergic M1-M2) were measured with quantitative receptor autoradiography using tritiated ligands. Measurements of fear-sensitized acoustic startle response (ASR; induced by footshocks), elevated plus maze (EPM) behavior and receptor binding studies showed differences between the strains except for AMPA and muscarinergic M2 receptors. Factor analysis revealed a Startle Factor with positive loadings of the density of serotonin and kainate receptors, and the amplitudes of the baseline and fear-sensitized ASRs. A second Anxiety-related Factor only correlated with the fear-sensitized ASR and anxiety parameters on the EPM but not receptor densities. There were also two General Activity Factors defined by (negative) correlations with entries to closed arms of the EPM. Because the density of NMDA and muscarinergic M1 receptors also correlated negatively with the two factors, these receptors had a positive effect on general activity. In contrast, correlations of GABAA, serotonin, and kainate receptors had the opposite sign as compared to closed arm entries. It is concluded that hereditary variations in the amygdala, particularly in kainate and serotonin receptors, play a role for the baseline and fear-sensitized ASR, whereas the general activity is influenced by many neurotransmitter receptor systems.

UR - http://www.scopus.com/inward/record.url?scp=0141645539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141645539&partnerID=8YFLogxK

U2 - 10.1016/S0166-4328(03)00107-4

DO - 10.1016/S0166-4328(03)00107-4

M3 - Article

C2 - 14529813

AN - SCOPUS:0141645539

VL - 145

SP - 145

EP - 159

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1-2

ER -