Assembly of a complete genome sequence for Gemmata obscuriglobus reveals a novel prokaryotic rRNA operon gene architecture

Josef D. Franke, Wilson R. Blomberg, Robert T. Todd, Robert W. Thomas, Anna Selmecki

Research output: Contribution to journalArticle

Abstract

Gemmata obscuriglobus is a Gram-negative bacterium with several intriguing biological features. Here, we present a complete, de novo whole genome assembly for G. obscuriglobus which consists of a single, circular 9 Mb chromosome, with no plasmids detected. The genome was annotated using the NCBI Prokaryotic Genome Annotation pipeline to generate common gene annotations. Analysis of the rRNA genes revealed three interesting features for a bacterium. First, linked G. obscuriglobus rrn operons have a unique gene order, 23S–5S–16S, compared to typical prokaryotic rrn operons (16S–23S–5S). Second, G. obscuriglobus rrn operons can either be linked or unlinked (a 16S gene is in a separate genomic location from a 23S and 5S gene pair). Third, all of the 23S genes (5 in total) have unique polymorphisms. Genome analysis of a different Gemmata species (SH-PL17), revealed a similar 23S–5S–16S gene order in all of its linked rrn operons and the presence of an unlinked operon. Together, our findings show that unique and rare features in Gemmata rrn operons among prokaryotes provide a means to better define the evolutionary relatedness of Gemmata species and the divergence time for different Gemmata species. Additionally, these rrn operon differences provide important insights into the rrn operon architecture of common ancestors of the planctomycetes.

LanguageEnglish (US)
Pages1-11
Number of pages11
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
DOIs
StateAccepted/In press - May 21 2018

Fingerprint

rRNA Operon
Operon
rRNA Genes
Genome
Gene Order
Genes
Molecular Sequence Annotation
Chromosomes, Human, Pair 9
Gram-Negative Bacteria
Plasmids
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Assembly of a complete genome sequence for Gemmata obscuriglobus reveals a novel prokaryotic rRNA operon gene architecture. / Franke, Josef D.; Blomberg, Wilson R.; Todd, Robert T.; Thomas, Robert W.; Selmecki, Anna.

In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 21.05.2018, p. 1-11.

Research output: Contribution to journalArticle

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