Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC β-lactamase, CMY-2

Johann D D Pitout, Mark Reisbig, Mike Mulvey, Linda Chui, Marie Louie, Larry Crowe, Deirdre L. Church, Sameer Elsayed, Dan Gregson, Rafiq Ahmed, Peter Tilley, Nancy D. Hanson

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Resistance to the extended-spectrum cephalosporins can occur in Salmonella species via the production of extended-spectrum and AmpC β-lactamases. We describe human infections with Salmonella enterica serotype Newport phage type 14 strains resistant to ceftazidime (CAZ) and cefoxitin (FOX) related to the handling of pet treats containing dried beef. These strains were isolated from five patients in Calgary, Alberta, Canada, during 2002 and were compared to a strain cultured from a commercial pet treat present at the property of one of the patients. The strains were resistant to FOX, CAZ, cefpodoxime, ampicillin, and chloramphenicol; intermediate resistant to ceftriaxone and cefotaxime; and sensitive to the aminoglycosides, ciprofloxacin, cefepime, and imipenem. Isoelectric focusing, multiplex PCR, and sequencing of the amplicons showed that all strains produced the plasmid-encoded AmpC β-lactamase, CMY-2. Restriction analysis of plasmid DNA following transformation demonstrated that blaCMY-2 was encoded on an approximately 140-kb plasmid. Pulsed-field gel electrophoresis showed the human and pet treat Salmonella strains to be highly related. This study is the first to implicate the transfer of multidrug-resistant Salmonella species through the handling of commercial pet treats containing animal products. In addition to documenting the first cases of human infection caused by CMY-2-producing S. enterica serotype Newport strains in Canada, this study illustrates the necessity of rapid and accurate laboratory-based surveillance in the identification of novel types of antimicrobial resistance.

Original languageEnglish
Pages (from-to)4578-4582
Number of pages5
JournalJournal of Clinical Microbiology
Volume41
Issue number10
DOIs
StatePublished - Oct 1 2003

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Salmonella enterica
Pets
Salmonella
Plasmids
Ceftazidime
cefpodoxime
Infection
Canada
Cefoxitin
Alberta
Cefotaxime
Ceftriaxone
Imipenem
Pulsed Field Gel Electrophoresis
Multiplex Polymerase Chain Reaction
Isoelectric Focusing
Aminoglycosides
Chloramphenicol
Cephalosporins
Ampicillin

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Microbiology

Cite this

Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC β-lactamase, CMY-2. / Pitout, Johann D D; Reisbig, Mark; Mulvey, Mike; Chui, Linda; Louie, Marie; Crowe, Larry; Church, Deirdre L.; Elsayed, Sameer; Gregson, Dan; Ahmed, Rafiq; Tilley, Peter; Hanson, Nancy D.

In: Journal of Clinical Microbiology, Vol. 41, No. 10, 01.10.2003, p. 4578-4582.

Research output: Contribution to journalArticle

Pitout, Johann D D ; Reisbig, Mark ; Mulvey, Mike ; Chui, Linda ; Louie, Marie ; Crowe, Larry ; Church, Deirdre L. ; Elsayed, Sameer ; Gregson, Dan ; Ahmed, Rafiq ; Tilley, Peter ; Hanson, Nancy D. / Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC β-lactamase, CMY-2. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 10. pp. 4578-4582.
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