Association between periodontitis and hyperlipidemia

Cause or effect?

Christopher W. Cutler, Edward A. Shinedling, Martha E. Nunn, Ravi Jotwani, Byung Ock Kim, Salvador Nares, Anthony M. Iacopino

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Background: Epidemiological studies suggest a relationship between periodontitis and coronary artery disease, but the mechanism has not been established. Recent studies in animals indicate that low dose endotoxin, as in a Gram-negative infection, can induce hyperlipidemia and myeloid cell hyperactivity. The association between periodontitis, systemic exposure to Porphyromonas gingivalis, lipopolysaccharides (LPS), and hyperlipidemia has not been examined in humans. Methods: Sera were obtained from 26 adult periodontitis patients and 25 healthy control (C) subjects selected from patients and staff. Serum antibodies against Porphyromonas gingivalis and its LPS were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting, respectively. Serum triglycerides (TG) and cholesterol (CHOL) were assayed by a commercial laboratory. The associations between AP and blood levels of TG, CHOL, and anti-P. gingivalis whole cells and LPS were examined by logistic regression analysis. Peripheral blood polymorphonuclear leukocytes (PMNs) from 6 healthy fasted donors were incubated with purified TG (0.1 mg/ml) for 2 hours at 37°C, stimulated with 100 ng/ml P. gingivalis LPS, and the release of IL-1β measured by ELISA. Results: The presence of periodontitis was significantly associated with age (odds ratio = 3.5, P = 0.04), elevated TG levels (odds ratio = 8.6, P = 0.0009), elevated CHOL levels (odds ratio = 7, P = 0.004), elevated ELISA titer (odds ratio = 35, P = 0.003) and reactivity with P. gingivalis LPS (odds ratio = 41, P = 0.001). PMNs from all 6 healthy patients released modest levels of IL-1β (10 to 60 pg/ml) when stimulated with 100 ng/ml P. gingivalis LPS. Addition of TG resulted in a significant increase (P

Original languageEnglish
Pages (from-to)1429-1434
Number of pages6
JournalJournal of Periodontology
Volume70
Issue number12
StatePublished - Dec 1999
Externally publishedYes

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Porphyromonas gingivalis
Periodontitis
Hyperlipidemias
Lipopolysaccharides
Triglycerides
Odds Ratio
Enzyme-Linked Immunosorbent Assay
Interleukin-1
Serum
Cholesterol
Chronic Periodontitis
Myeloid Cells
Hypercholesterolemia
Endotoxins
Interleukin-10
Epidemiologic Studies
Coronary Artery Disease
Neutrophils
Logistic Models
Western Blotting

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Cutler, C. W., Shinedling, E. A., Nunn, M. E., Jotwani, R., Kim, B. O., Nares, S., & Iacopino, A. M. (1999). Association between periodontitis and hyperlipidemia: Cause or effect? Journal of Periodontology, 70(12), 1429-1434.

Association between periodontitis and hyperlipidemia : Cause or effect? / Cutler, Christopher W.; Shinedling, Edward A.; Nunn, Martha E.; Jotwani, Ravi; Kim, Byung Ock; Nares, Salvador; Iacopino, Anthony M.

In: Journal of Periodontology, Vol. 70, No. 12, 12.1999, p. 1429-1434.

Research output: Contribution to journalArticle

Cutler, CW, Shinedling, EA, Nunn, ME, Jotwani, R, Kim, BO, Nares, S & Iacopino, AM 1999, 'Association between periodontitis and hyperlipidemia: Cause or effect?', Journal of Periodontology, vol. 70, no. 12, pp. 1429-1434.
Cutler CW, Shinedling EA, Nunn ME, Jotwani R, Kim BO, Nares S et al. Association between periodontitis and hyperlipidemia: Cause or effect? Journal of Periodontology. 1999 Dec;70(12):1429-1434.
Cutler, Christopher W. ; Shinedling, Edward A. ; Nunn, Martha E. ; Jotwani, Ravi ; Kim, Byung Ock ; Nares, Salvador ; Iacopino, Anthony M. / Association between periodontitis and hyperlipidemia : Cause or effect?. In: Journal of Periodontology. 1999 ; Vol. 70, No. 12. pp. 1429-1434.
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