Association of IS5 with divergent tandem bla CMY-2 genes in clinical isolates of Escherichia coli

Philip M. Kurpiel, Nancy D. Hanson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To characterize a unique tandem bla CMY-2 gene arrangement found in two non-identical clinical strains of Escherichia coli. Methods: Both plasmid and chromosomal DNA were evaluated using PFGE, restriction digest analysis, plasmid profiling and Southern hybridization. bla CMY-2gene expression and gene copy number were evaluated by realtime PCR. Susceptibilities to selected β-lactam antibiotics were determined by agar dilution. Results: A tandem arrangement for bla CMY-2 was identified in both isolates and was the only arrangement for bla CMY-2 observed. These isolates had distinct PFGE and plasmid profiles. Each strain exhibited 2-fold higher bla CMY-2mRNA expression and up to 8-fold lower β-lactam susceptibility compared with a strain with a single copy of bla CMY-2. Conclusion: This is the first report of IS5 being associated with tandem bla CMY-2. IS5 has previously been associated with antibiotic resistance through tandem gene amplification. The unique tandem arrangement provides a mechanism for increased bla CMY-2expression.

Original languageEnglish
Article numberdkr212
Pages (from-to)1734-1738
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume66
Issue number8
DOIs
StatePublished - Aug 2011

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Escherichia coli
Lactams
Plasmids
Genes
Gene Order
Gene Dosage
Gene Amplification
Microbial Drug Resistance
Agar
beta-lactamase CMY-2
Anti-Bacterial Agents
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Association of IS5 with divergent tandem bla CMY-2 genes in clinical isolates of Escherichia coli. / Kurpiel, Philip M.; Hanson, Nancy D.

In: Journal of Antimicrobial Chemotherapy, Vol. 66, No. 8, dkr212, 08.2011, p. 1734-1738.

Research output: Contribution to journalArticle

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