Association of tumor development with increased cellular proliferation and transgene overexpression, but not c-Ha-ras mutations, in v-Ha-ras transgenic Tg.AC mice

Laura A. Hansen, Carol S. Trempus, Joel F. Mahler, Raymond W. Tennant

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The transgenic mouse line Tg.AC carries a v-Ha-ras gene fused to a fetal (ξ) globin promoter and uniquely responds to chemical carcinogens and tumor promoters by the induction of epidermal papillomas. Although the transgene was not constitutively expressed in non-tumor-bearing tissues, expression was induced by exposure to selected chemicals. Tg.AC transgenic mice on the FVB/N background developed occasional spontaneous tumors, including odontomas, squamous cell carcinoma of the salivary gland, leukemias and a rare ovarian yolk sac carcinoma. Both spontaneous and induced tumors are associated with expression of the transgene and, as determined by in situ hybridization, transgene expression is localized to proliferative areas of the tumors. Sequence analysis of the endogenous c-Ha-ras gene in both induced and spontaneous tumors revealed no mutations in codons 12, 59 or 61. These results suggest that expression of the v-Ha-ras transgene induces proliferation of specific cells in diverse tissues which then acquire neoplastic properties.

Original languageEnglish
Pages (from-to)1825-1833
Number of pages9
JournalCarcinogenesis
Volume17
Issue number9
StatePublished - Sep 1996
Externally publishedYes

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Transgenes
Cell Proliferation
Mutation
ras Genes
Carcinogens
Transgenic Mice
Neoplasms
Odontoma
Yolk Sac
Globins
Papilloma
Salivary Glands
Codon
In Situ Hybridization
Sequence Analysis
Squamous Cell Carcinoma
Leukemia
Carcinoma

All Science Journal Classification (ASJC) codes

  • Cancer Research

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Association of tumor development with increased cellular proliferation and transgene overexpression, but not c-Ha-ras mutations, in v-Ha-ras transgenic Tg.AC mice. / Hansen, Laura A.; Trempus, Carol S.; Mahler, Joel F.; Tennant, Raymond W.

In: Carcinogenesis, Vol. 17, No. 9, 09.1996, p. 1825-1833.

Research output: Contribution to journalArticle

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