Asteropsin A

An unusual cystine-crosslinked peptide from porifera enhances neuronal Ca2 + influx

Huayue Li, John J. Bowling, Frank R. Fronczek, Jongki Hong, Sairam V. Jabba, Thomas F. Murray, Nam Chul Ha, Mark T. Hamann, Jee H. Jung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background Herein we report the discovery of a cystine-crosslinked peptide from Porifera along with high-quality spatial details accompanied by the description of its unique effect on neuronal calcium influx. Methods Asteropsin A (ASPA) was isolated from the marine sponge Asteropus sp., and its structure was independently determined using X-ray crystallography (0.87 Å) and solution NMR spectroscopy. Results An N-terminal pyroglutamate modification, uncommon cis proline conformations, and absence of basic residues helped distinguish ASPA from other cystine-crosslinked knot peptides. ASPA enhanced Ca2 + influx in murine cerebrocortical neuron cells following the addition of the Na+ channel activator veratridine but did not modify the oscillation frequency or amplitude of neuronal Ca2 + currents alone. Allosterism at neurotoxin site 2 was not observed, suggesting an alternative to the known Na+ channel interaction. Conclusions Together with a distinct biological activity, the origin of ASPA suggests a new subclass of cystine-rich knot peptides associated with Porifera. General significance The discovery of ASPA represents a distinctive addition to an emerging subclass of cystine-crosslinked knot peptides from Porifera.

Original languageEnglish
Pages (from-to)2591-2599
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1830
Issue number3
DOIs
StatePublished - Mar 2013

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Cystine
Porifera
Peptides
Pyrrolidonecarboxylic Acid
Veratridine
X ray crystallography
X Ray Crystallography
Neurotoxins
Bioactivity
Proline
Nuclear magnetic resonance spectroscopy
Neurons
Conformations
Magnetic Resonance Spectroscopy
asteropsin A
Calcium

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Asteropsin A : An unusual cystine-crosslinked peptide from porifera enhances neuronal Ca2 + influx. / Li, Huayue; Bowling, John J.; Fronczek, Frank R.; Hong, Jongki; Jabba, Sairam V.; Murray, Thomas F.; Ha, Nam Chul; Hamann, Mark T.; Jung, Jee H.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1830, No. 3, 03.2013, p. 2591-2599.

Research output: Contribution to journalArticle

Li, Huayue ; Bowling, John J. ; Fronczek, Frank R. ; Hong, Jongki ; Jabba, Sairam V. ; Murray, Thomas F. ; Ha, Nam Chul ; Hamann, Mark T. ; Jung, Jee H. / Asteropsin A : An unusual cystine-crosslinked peptide from porifera enhances neuronal Ca2 + influx. In: Biochimica et Biophysica Acta - General Subjects. 2013 ; Vol. 1830, No. 3. pp. 2591-2599.
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