Attachment of mesophilic aeromonads to cultured mammalian cells

Richard B. Clark, Floyd C. Knoop, Patricia J. Padgitt, Daniel H. Hu, Jane D. Wong, J. Michael Janda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The interaction between mesophilic aeromonads and cultured mouse adrenal cells was examined. Preliminary experiments indicated that aeromonad attachment was dependent upon inoculum size, incubation time, and incubation temperature. Optimal attachment was observed after 30 min of incubation at 37°C with an inoculum size of 1×107 CFU. Heat-killed and formalin-treated organisms did not attach to the cultured cell system. The attachment of aeromonads to the mammalian cell surface was confirmed by light and scanning electron microscopy. Aeromonad attachment correlated both with the presence of pili and the specific aeromonad species, but not with hydrophobicity or the ability to autoagglutinate. Piliated strains were more likely to show high or moderate attachment. Aeromonas sobria, A. hydrophila, and A. veronii showed a greater ability to bind adrenal cells than did A. caviae. Removal of the pili from two A. sobria isolates markedly reduced their attachment. In contrast, one A. hydrophila isolate was strongly adherent after the removal of pili. The hemagglutination patterns produced by A. sobria and the other aeromonad species were distinctly different, but potentially predictive of the ability of aeromonads to attach to cultured mouse adrenal cells. These studies indicate that multiple mechanisms are important for the attachment of mesophilic aeromonads to mammalian cells. This model may prove useful for studying the pathogenesis of aeromonad infections.

Original languageEnglish (US)
Pages (from-to)97-102
Number of pages6
JournalCurrent Microbiology
Volume19
Issue number2
DOIs
StatePublished - Aug 1 1989

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Cultured Cells
Aeromonas
Hemagglutination
Hydrophobic and Hydrophilic Interactions
Electron Scanning Microscopy
Formaldehyde
Hot Temperature
Light
Temperature
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Clark, R. B., Knoop, F. C., Padgitt, P. J., Hu, D. H., Wong, J. D., & Janda, J. M. (1989). Attachment of mesophilic aeromonads to cultured mammalian cells. Current Microbiology, 19(2), 97-102. https://doi.org/10.1007/BF01570575

Attachment of mesophilic aeromonads to cultured mammalian cells. / Clark, Richard B.; Knoop, Floyd C.; Padgitt, Patricia J.; Hu, Daniel H.; Wong, Jane D.; Janda, J. Michael.

In: Current Microbiology, Vol. 19, No. 2, 01.08.1989, p. 97-102.

Research output: Contribution to journalArticle

Clark, RB, Knoop, FC, Padgitt, PJ, Hu, DH, Wong, JD & Janda, JM 1989, 'Attachment of mesophilic aeromonads to cultured mammalian cells', Current Microbiology, vol. 19, no. 2, pp. 97-102. https://doi.org/10.1007/BF01570575
Clark, Richard B. ; Knoop, Floyd C. ; Padgitt, Patricia J. ; Hu, Daniel H. ; Wong, Jane D. ; Janda, J. Michael. / Attachment of mesophilic aeromonads to cultured mammalian cells. In: Current Microbiology. 1989 ; Vol. 19, No. 2. pp. 97-102.
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