Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1

Zhenping Ming, Yang Wang, Ai Yu Gong, Xin Tian Zhang, Min Li, Ting Chen, Nicholas W. Mathy, Juliane K. Strauss-Soukup, Xian-Ming Chen

Research output: Contribution to journalArticle

Abstract

Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.

LanguageEnglish (US)
Pages1336-1347
Number of pages12
JournalJournal of Infectious Diseases
Volume218
Issue number8
DOIs
StatePublished - Sep 8 2018

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Cryptosporidium parvum
Parasitic Diseases
Cell Movement
Epithelial Cells
RNA
Cryptosporidium
Proteins
Infection
Parasites
Cell Migration Inhibition
Cryptosporidiosis
Transcriptional Activation

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1. / Ming, Zhenping; Wang, Yang; Gong, Ai Yu; Zhang, Xin Tian; Li, Min; Chen, Ting; Mathy, Nicholas W.; Strauss-Soukup, Juliane K.; Chen, Xian-Ming.

In: Journal of Infectious Diseases, Vol. 218, No. 8, 08.09.2018, p. 1336-1347.

Research output: Contribution to journalArticle

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abstract = "Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.",
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