Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1

Zhenping Ming, Yang Wang, Ai Yu Gong, Xin Tian Zhang, Min Li, Ting Chen, Nicholas W. Mathy, Juliane K. Strauss-Soukup, Xian-Ming Chen

Research output: Contribution to journalArticle

1 Scopus citations


Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.

Original languageEnglish (US)
Pages (from-to)1336-1347
Number of pages12
JournalJournal of Infectious Diseases
Issue number8
Publication statusPublished - Sep 8 2018


All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this