Abstract
Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.
Original language | English (US) |
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Pages (from-to) | 1336-1347 |
Number of pages | 12 |
Journal | Journal of Infectious Diseases |
Volume | 218 |
Issue number | 8 |
DOIs | |
State | Published - Sep 8 2018 |
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Infectious Diseases