Abstract
Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1336-1347 |
| Number of pages | 12 |
| Journal | Journal of Infectious Diseases |
| Volume | 218 |
| Issue number | 8 |
| DOIs | |
| State | Published - Sep 8 2018 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Infectious Diseases
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Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1. / Ming, Zhenping; Wang, Yang; Gong, Ai Yu; Zhang, Xin Tian; Li, Min; Chen, Ting; Mathy, Nicholas W.; Strauss-Soukup, Juliane K.; Chen, Xian-Ming.
In: Journal of Infectious Diseases, Vol. 218, No. 8, 08.09.2018, p. 1336-1347.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1
AU - Ming, Zhenping
AU - Wang, Yang
AU - Gong, Ai Yu
AU - Zhang, Xin Tian
AU - Li, Min
AU - Chen, Ting
AU - Mathy, Nicholas W.
AU - Strauss-Soukup, Juliane K.
AU - Chen, Xian-Ming
PY - 2018/9/8
Y1 - 2018/9/8
N2 - Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.
AB - Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.
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U2 - 10.1093/infdis/jiy299
DO - 10.1093/infdis/jiy299
M3 - Article
C2 - 30052999
AN - SCOPUS:85054951988
VL - 218
SP - 1336
EP - 1347
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 8
ER -