Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1

Zhenping Ming; Yang Wang; Ai Yu Gong; Xin Tian Zhang; Min Li; Ting Chen; Nicholas W. Mathy; Juliane K. Strauss-Soukup; Xian Ming Chen

doi:10.1093/infdis/jiy299

Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1

Zhenping Ming, Yang Wang, Ai Yu Gong, Xin Tian Zhang, Min Li, Ting Chen, Nicholas W. Mathy, Juliane K. Strauss-Soukup, Xian Ming Chen

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.

Original language	English (US)
Pages (from-to)	1336-1347
Number of pages	12
Journal	Journal of Infectious Diseases
Volume	218
Issue number	8
DOIs	https://doi.org/10.1093/infdis/jiy299
State	Published - Sep 8 2018

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/jiy299

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Ming, Z., Wang, Y., Gong, A. Y., Zhang, X. T., Li, M., Chen, T., Mathy, N. W., Strauss-Soukup, J. K., & Chen, X. M. (2018). Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1. Journal of Infectious Diseases, 218(8), 1336-1347. https://doi.org/10.1093/infdis/jiy299

Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1. / Ming, Zhenping; Wang, Yang; Gong, Ai Yu; Zhang, Xin Tian; Li, Min; Chen, Ting; Mathy, Nicholas W.; Strauss-Soukup, Juliane K.; Chen, Xian Ming.

In: Journal of Infectious Diseases, Vol. 218, No. 8, 08.09.2018, p. 1336-1347.

Research output: Contribution to journal › Article › peer-review

Ming, Z, Wang, Y, Gong, AY, Zhang, XT, Li, M, Chen, T, Mathy, NW, Strauss-Soukup, JK & Chen, XM 2018, 'Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1', Journal of Infectious Diseases, vol. 218, no. 8, pp. 1336-1347. https://doi.org/10.1093/infdis/jiy299

Ming, Zhenping ; Wang, Yang ; Gong, Ai Yu ; Zhang, Xin Tian ; Li, Min ; Chen, Ting ; Mathy, Nicholas W. ; Strauss-Soukup, Juliane K. ; Chen, Xian Ming. / Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1. In: Journal of Infectious Diseases. 2018 ; Vol. 218, No. 8. pp. 1336-1347.

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title = "Attenuation of intestinal epithelial cell migration during cryptosporidium parvum infection involves parasite Cdg7-FLc-1030 RNA-Mediated induction and release of dickkopf-1",

abstract = "Intestinal infection by Cryptosporidium is known to cause epithelial cell migration disorder but the underlying mechanisms are unclear. Previous studies demonstrated that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using multiple models of intestinal cryptosporidiosis, we report here that C. parvum infection induces expression and release of the dickkopf protein 1 (Dkk1) from intestinal epithelial cells. Delivery of parasite Cdg7-FLc-1030 RNA to intestinal epithelial cells triggers transactivation of host Dkk1 gene during C. parvum infection. Release of Dkk1 is involved in C. parvum-induced inhibition of cell migration of epithelial cells, including noninfected bystander cells. Moreover, Dkk1-mediated suppression of host cell migration during C. parvum infection involves inhibition of Cdc42/Par6 signaling. Our data support the hypothesis that attenuation of intestinal epithelial cell migration during Cryptosporidium infection involves parasite Cdg7-FLc-1030 RNA-mediated induction and release of Dkk1 from infected cells.",

author = "Zhenping Ming and Yang Wang and Gong, {Ai Yu} and Zhang, {Xin Tian} and Min Li and Ting Chen and Mathy, {Nicholas W.} and Strauss-Soukup, {Juliane K.} and Chen, {Xian Ming}",

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