Avian pancreatic polypeptide fragments refold to native aPP conformation when combined in solution: A CD and VCD study

Jeffrey Copps, Richard F. Murphy, Sándor Lovas

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

An equimolar mixture of avion pancreatic polypeptide (aPP) fragments aPP(1-11)-NH2 and Ac-aPP(12-36) had an electronic circular dichroism (ECD) spectrum that was similar to that of whole aPP in H2O and even more so in 30% (v/v) trifluoroethanol (TFE) in 15 mM Na2HPO 4, but was different from the sum of the spectra of the individual fragments. The vibrational circular dichroism (VCD) spectrum of the combined fragments in 30% (v/v) TFE in 15 mM Na2HPO4 in D 2O was also similar to that of the intact aPP and unlike the sum of the VCD spectra of the fragments. The interaction of these fragments is thus sufficient to support the conformation of whole aPP. This study demonstrates that VCD, in combination with ECD, is useful for the study of protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalBiopolymers
Volume83
Issue number1
DOIs
StatePublished - Sep 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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