Behavioral analysis of NR2C knockout mouse reveals deficit in acquisition of conditioned fear and working memory

Brandon G. Hillman, Subhash C. Gupta, Dustin Stairs, Andres Buonanno, Shashank M. Dravid

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

N-methyl- D-aspartate (NMDA) receptors play an important role in excitatory neurotransmission and mediate synaptic plasticity associated with learning and memory. NMDA receptors are composed of two NR1 and two NR2 subunits and the identity of the NR2 subunit confers unique electrophysiologic and pharmacologic properties to the receptor. The precise role of NR2C-containing receptors in vivo is poorly understood. We have performed a battery of behavioral tests on NR2C knockout/. nβ-galactosidase knock-in mice and found no difference in spontaneous activity, basal anxiety, forced-swim immobility, novel object recognition, pain sensitivity and reference memory in comparison to wildtype counterparts. However, NR2C knockout mice were found to exhibit deficits in fear acquisition and working memory compared to wildtype mice. Deficit in fear acquisition correlated with lack of fear conditioning-induced plasticity at the thalamo-amygdala synapse. These findings suggest a unique role of NR2C-containing receptors in associative and executive learning representing a novel therapeutic target for deficits in cognition.

Original languageEnglish
Pages (from-to)404-414
Number of pages11
JournalNeurobiology of Learning and Memory
Volume95
Issue number4
DOIs
StatePublished - May 2011

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Short-Term Memory
Knockout Mice
Fear
N-Methyl-D-Aspartate Receptors
Learning
Galactosidases
Neuronal Plasticity
Amygdala
Synaptic Transmission
Synapses
Cognition
Anxiety
Pain
Therapeutics

All Science Journal Classification (ASJC) codes

  • Behavioral Neuroscience
  • Cognitive Neuroscience
  • Experimental and Cognitive Psychology

Cite this

Behavioral analysis of NR2C knockout mouse reveals deficit in acquisition of conditioned fear and working memory. / Hillman, Brandon G.; Gupta, Subhash C.; Stairs, Dustin; Buonanno, Andres; Dravid, Shashank M.

In: Neurobiology of Learning and Memory, Vol. 95, No. 4, 05.2011, p. 404-414.

Research output: Contribution to journalArticle

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