Binding of NF-kappaB p65 subunit to the promoter elements is involved in LPS-induced transactivation of miRNA genes in human biliary epithelial cells

Rui Zhou, Guoku Hu, Ai Y. Gong, Xian-Ming Chen

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

The majority of human miRNA genes is transcribed by polymerase II and can be classified as class II genes similar to protein-coding genes. Whereas current research on miRNAs has focused on the physiological and pathological functions, the molecular mechanisms underlying their transcriptional regulation are largely unknown. We recently reported that lipopolysaccharide (LPS) alters mature miRNA expression profile in human biliary epithelial cells. In this study, we tested the role of transcription factor NF-kB in LPS-induced transcription of select miRNA genes. Of the majority of LPS-up-regulated mature miRNAs in cultured human biliary epithelial cells, potential NF-kB binding sites were identified in the putative promoter elements of their corresponding genes. Inhibition of NF-B activation by SC-514, an IKK2 inhibitor, blocked LPS-induced up-regulation of a subset of pri-miRNAs, including pri-miR17-92, pri-miR-125b-1, pri-miR-21, pri-miR23b-27b-24-1, pri-miR-30b, pri-miR-130a and pri-miR-29a. Moreover, direct binding of NF-kB p65 subunit to the promoter elements of mir-17-92, mir-125b-1, mir-21, mir-23b-27b-24-1, mir-30b and mir-130a genes was identified by chromatin immunoprecipitation analysis and confirmed by the luciferase reporter assay. Thus, a subset of miRNA genes is regulated in human biliary epithelial cells through NF-kB activation induced by LPS, suggesting a role of the NF-kB pathway in the transcriptional regulation of miRNA genes.

Original languageEnglish
Article numbergkq056
Pages (from-to)3222-3232
Number of pages11
JournalNucleic Acids Research
Volume38
Issue number10
DOIs
StatePublished - Feb 9 2010

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NF-kappa B
MicroRNAs
Transcriptional Activation
Lipopolysaccharides
Epithelial Cells
Genes
MHC Class II Genes
Chromatin Immunoprecipitation
Luciferases
Up-Regulation
Binding Sites
Research

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Binding of NF-kappaB p65 subunit to the promoter elements is involved in LPS-induced transactivation of miRNA genes in human biliary epithelial cells. / Zhou, Rui; Hu, Guoku; Gong, Ai Y.; Chen, Xian-Ming.

In: Nucleic Acids Research, Vol. 38, No. 10, gkq056, 09.02.2010, p. 3222-3232.

Research output: Contribution to journalArticle

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