Abstract
Conditions required for in vitro bonding of14C-ofloxacin (OFX) to polytetrafluoroethylene suture (PTFE) were investigated. Bonding of OFX to PTFE was enhanced 22- and 75-fold in the presence of the cationic surfactant benzalkonium chloride (BAC) or silver and organic solvents (S/O), respectively. The optimal temperature and time of incubation for maximum OFX bonding was 37°C for 1 h (BAC) and 37°C for 25 min (S/O). BAC- and S/O-treated PTFE bound 3.94 nmoles and 17.79 nmoles OFX/cm suture. Prolonged in vitro incubation of OFX/PTFE for 7 days at 37°C in the presence of serum or saline resulted in 50% OFX retention. In vivo studies on OFX/PTFE placed into canine carotid arteries showed 8% OFX retention. The intravenous infusion of coagulase-positive Staphylococcus aureus into OFX/PTFE animals resulted in colonization. Although significant in vitro bonding of OFX to PTFE was observed, in vivo studies demonstrate an accelerated release and inferior OFX/PTFE activity against microbial colonization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-30 |
| Number of pages | 4 |
| Journal | Current Microbiology |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1990 |
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All Science Journal Classification (ASJC) codes
- Microbiology
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Bonding of ofloxacin to polytetrafluoroethylene suture and colonization by Staphylococcus aureus. / Knoop, Floyd C.; Dworzack, David L.; Martig, Robert J.; Sterpetti, Antonio V.; Bailey, Robert T.; Schultz, Richard D.
In: Current Microbiology, Vol. 20, No. 1, 01.01.1990, p. 27-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bonding of ofloxacin to polytetrafluoroethylene suture and colonization by Staphylococcus aureus
AU - Knoop, Floyd C.
AU - Dworzack, David L.
AU - Martig, Robert J.
AU - Sterpetti, Antonio V.
AU - Bailey, Robert T.
AU - Schultz, Richard D.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - Conditions required for in vitro bonding of14C-ofloxacin (OFX) to polytetrafluoroethylene suture (PTFE) were investigated. Bonding of OFX to PTFE was enhanced 22- and 75-fold in the presence of the cationic surfactant benzalkonium chloride (BAC) or silver and organic solvents (S/O), respectively. The optimal temperature and time of incubation for maximum OFX bonding was 37°C for 1 h (BAC) and 37°C for 25 min (S/O). BAC- and S/O-treated PTFE bound 3.94 nmoles and 17.79 nmoles OFX/cm suture. Prolonged in vitro incubation of OFX/PTFE for 7 days at 37°C in the presence of serum or saline resulted in 50% OFX retention. In vivo studies on OFX/PTFE placed into canine carotid arteries showed 8% OFX retention. The intravenous infusion of coagulase-positive Staphylococcus aureus into OFX/PTFE animals resulted in colonization. Although significant in vitro bonding of OFX to PTFE was observed, in vivo studies demonstrate an accelerated release and inferior OFX/PTFE activity against microbial colonization.
AB - Conditions required for in vitro bonding of14C-ofloxacin (OFX) to polytetrafluoroethylene suture (PTFE) were investigated. Bonding of OFX to PTFE was enhanced 22- and 75-fold in the presence of the cationic surfactant benzalkonium chloride (BAC) or silver and organic solvents (S/O), respectively. The optimal temperature and time of incubation for maximum OFX bonding was 37°C for 1 h (BAC) and 37°C for 25 min (S/O). BAC- and S/O-treated PTFE bound 3.94 nmoles and 17.79 nmoles OFX/cm suture. Prolonged in vitro incubation of OFX/PTFE for 7 days at 37°C in the presence of serum or saline resulted in 50% OFX retention. In vivo studies on OFX/PTFE placed into canine carotid arteries showed 8% OFX retention. The intravenous infusion of coagulase-positive Staphylococcus aureus into OFX/PTFE animals resulted in colonization. Although significant in vitro bonding of OFX to PTFE was observed, in vivo studies demonstrate an accelerated release and inferior OFX/PTFE activity against microbial colonization.
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U2 - 10.1007/BF02094020
DO - 10.1007/BF02094020
M3 - Article
VL - 20
SP - 27
EP - 30
JO - Current Microbiology
JF - Current Microbiology
SN - 0343-8651
IS - 1
ER -