Bonding of ofloxacin to polytetrafluoroethylene suture and colonization by Staphylococcus aureus

Floyd C. Knoop, David L. Dworzack, Robert J. Martig, Antonio V. Sterpetti, Robert T. Bailey, Richard D. Schultz

Research output: Contribution to journalArticlepeer-review


Conditions required for in vitro bonding of14C-ofloxacin (OFX) to polytetrafluoroethylene suture (PTFE) were investigated. Bonding of OFX to PTFE was enhanced 22- and 75-fold in the presence of the cationic surfactant benzalkonium chloride (BAC) or silver and organic solvents (S/O), respectively. The optimal temperature and time of incubation for maximum OFX bonding was 37°C for 1 h (BAC) and 37°C for 25 min (S/O). BAC- and S/O-treated PTFE bound 3.94 nmoles and 17.79 nmoles OFX/cm suture. Prolonged in vitro incubation of OFX/PTFE for 7 days at 37°C in the presence of serum or saline resulted in 50% OFX retention. In vivo studies on OFX/PTFE placed into canine carotid arteries showed 8% OFX retention. The intravenous infusion of coagulase-positive Staphylococcus aureus into OFX/PTFE animals resulted in colonization. Although significant in vitro bonding of OFX to PTFE was observed, in vivo studies demonstrate an accelerated release and inferior OFX/PTFE activity against microbial colonization.

Original languageEnglish (US)
Pages (from-to)27-30
Number of pages4
JournalCurrent Microbiology
Issue number1
StatePublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology


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