Bradykinin-related peptides and tryptophyllins in the skin secretions of the most primitive extant frog, Ascaphus truei

J. Michael Conlon, Thierry Jouenne, Pascal Cosette, David Cosquer, Hubert Vaudry, Christopher K. Taylor, Peter W. Abel

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The tailed frog Ascaphus truei occupies a unique position in phylogeny as the most primitive extant anuran and is regarded as the sister taxon to the clade of all other living frogs. A previous study led to the isolation of eight antimicrobial peptides, termed ascaphins, from norepinephrine-stimulated skin secretions. Peptidomic analysis (HPLC separation followed by MALDI mass spectrometry and Edman degradation) of these secretions has led to the identification and structural characterization of 13 additional peptides present in relatively high concentration. In addition to bradykinin (BK; RPPGFSPFR), a C-terminally extended bradykinin (peptide RD-11; RPPGFSPFRVD), a bradykinin-like peptide (peptide AR-10; APVPGLSPFR), and a C-terminally extended form of this peptide (peptide AV-12; APVPGLSPFRVV) were obtained in pure form. These peptides produced concentration-dependent relaxation of precontracted mouse tracheal rings with a rank order of potency of BK > RD-11 > AR-10 > AV-12 but only RD-11 caused the same maximal relaxation as bradykinin. Four small peptides were also isolated from the skin secretions that contain the Pro-Trp motif that is a characteristic of the tryptophyllin family of peptides previously identified in skins of frogs of the family Hylidae. The data show that the synthesis of dermal peptides that may play a role in defense against predators arose early in the evolution of anurans.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume143
Issue number2
DOIs
StatePublished - Sep 1 2005

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bradykinin
Bradykinin
skin (animal)
Anura
frogs
secretion
peptides
Skin
Peptides
tryptophyllins
matrix-assisted laser desorption-ionization mass spectrometry
antimicrobial peptides
Hylidae
norepinephrine
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Phylogeny
high performance liquid chromatography
Mass Spectrometry
Norepinephrine
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Endocrinology

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Bradykinin-related peptides and tryptophyllins in the skin secretions of the most primitive extant frog, Ascaphus truei. / Conlon, J. Michael; Jouenne, Thierry; Cosette, Pascal; Cosquer, David; Vaudry, Hubert; Taylor, Christopher K.; Abel, Peter W.

In: General and Comparative Endocrinology, Vol. 143, No. 2, 01.09.2005, p. 193-199.

Research output: Contribution to journalArticle

Conlon, J. Michael ; Jouenne, Thierry ; Cosette, Pascal ; Cosquer, David ; Vaudry, Hubert ; Taylor, Christopher K. ; Abel, Peter W. / Bradykinin-related peptides and tryptophyllins in the skin secretions of the most primitive extant frog, Ascaphus truei. In: General and Comparative Endocrinology. 2005 ; Vol. 143, No. 2. pp. 193-199.
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