Abstract
Birds serve as reservoirs for at least 10 arthropod-borne viruses, yet specific immune responses of birds to arboviral infections are relatively unknown. Here, adult House Sparrows were inoculated with an arboviral alphavirus, Buggy Creek virus (BCRV), or saline, and euthanized between 1 and 3 days postinoculation. Virological dynamics and gene expression dynamics were investigated. Birds did not develop viremia postinoculation, but cytopathic virus was found in the skeletal muscle and spleen of birds 1 and 3 days postinoculation (DPI). Viral RNA was detected in the blood of BCRV-infected birds 1 and 2 DPI, in oral swabs 1-3 DPI, and in brain, heart, skeletal muscle, and spleen 1-3 DPI. Multiple genes were significantly upregulated following BCRV infection, including pattern recognition receptors (TLR7, TLR15, RIG-1), type I interferon (IFN-α), and type II interferon (IFN-γ). This is the first study to report avian immunological gene expression profiles following an arboviral infection.
| Original language | English |
|---|---|
| Pages (from-to) | 439-446 |
| Number of pages | 8 |
| Journal | Vector-Borne and Zoonotic Diseases |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2014 |
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All Science Journal Classification (ASJC) codes
- Infectious Diseases
- Microbiology
- Virology
- Medicine(all)
Cite this
Buggy creek virus (Togaviridae : Alphavirus) upregulates expression of pattern recognition receptors and interferons in house sparrows (Passer domesticus). / Fassbinder-Orth, Carol; Barak, Virginia A.; Rainwater, Ellecia L.; Altrichter, Ashley M.
In: Vector-Borne and Zoonotic Diseases, Vol. 14, No. 6, 01.06.2014, p. 439-446.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Buggy creek virus (Togaviridae
T2 - Alphavirus) upregulates expression of pattern recognition receptors and interferons in house sparrows (Passer domesticus)
AU - Fassbinder-Orth, Carol
AU - Barak, Virginia A.
AU - Rainwater, Ellecia L.
AU - Altrichter, Ashley M.
PY - 2014/6/1
Y1 - 2014/6/1
N2 - Birds serve as reservoirs for at least 10 arthropod-borne viruses, yet specific immune responses of birds to arboviral infections are relatively unknown. Here, adult House Sparrows were inoculated with an arboviral alphavirus, Buggy Creek virus (BCRV), or saline, and euthanized between 1 and 3 days postinoculation. Virological dynamics and gene expression dynamics were investigated. Birds did not develop viremia postinoculation, but cytopathic virus was found in the skeletal muscle and spleen of birds 1 and 3 days postinoculation (DPI). Viral RNA was detected in the blood of BCRV-infected birds 1 and 2 DPI, in oral swabs 1-3 DPI, and in brain, heart, skeletal muscle, and spleen 1-3 DPI. Multiple genes were significantly upregulated following BCRV infection, including pattern recognition receptors (TLR7, TLR15, RIG-1), type I interferon (IFN-α), and type II interferon (IFN-γ). This is the first study to report avian immunological gene expression profiles following an arboviral infection.
AB - Birds serve as reservoirs for at least 10 arthropod-borne viruses, yet specific immune responses of birds to arboviral infections are relatively unknown. Here, adult House Sparrows were inoculated with an arboviral alphavirus, Buggy Creek virus (BCRV), or saline, and euthanized between 1 and 3 days postinoculation. Virological dynamics and gene expression dynamics were investigated. Birds did not develop viremia postinoculation, but cytopathic virus was found in the skeletal muscle and spleen of birds 1 and 3 days postinoculation (DPI). Viral RNA was detected in the blood of BCRV-infected birds 1 and 2 DPI, in oral swabs 1-3 DPI, and in brain, heart, skeletal muscle, and spleen 1-3 DPI. Multiple genes were significantly upregulated following BCRV infection, including pattern recognition receptors (TLR7, TLR15, RIG-1), type I interferon (IFN-α), and type II interferon (IFN-γ). This is the first study to report avian immunological gene expression profiles following an arboviral infection.
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U2 - 10.1089/vbz.2013.1531
DO - 10.1089/vbz.2013.1531
M3 - Article
C2 - 24866749
AN - SCOPUS:84902506417
VL - 14
SP - 439
EP - 446
JO - Vector-Borne and Zoonotic Diseases
JF - Vector-Borne and Zoonotic Diseases
SN - 1530-3667
IS - 6
ER -