Buggy creek virus (Togaviridae

Alphavirus) upregulates expression of pattern recognition receptors and interferons in house sparrows (Passer domesticus)

Carol Fassbinder-Orth, Virginia A. Barak, Ellecia L. Rainwater, Ashley M. Altrichter

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Birds serve as reservoirs for at least 10 arthropod-borne viruses, yet specific immune responses of birds to arboviral infections are relatively unknown. Here, adult House Sparrows were inoculated with an arboviral alphavirus, Buggy Creek virus (BCRV), or saline, and euthanized between 1 and 3 days postinoculation. Virological dynamics and gene expression dynamics were investigated. Birds did not develop viremia postinoculation, but cytopathic virus was found in the skeletal muscle and spleen of birds 1 and 3 days postinoculation (DPI). Viral RNA was detected in the blood of BCRV-infected birds 1 and 2 DPI, in oral swabs 1-3 DPI, and in brain, heart, skeletal muscle, and spleen 1-3 DPI. Multiple genes were significantly upregulated following BCRV infection, including pattern recognition receptors (TLR7, TLR15, RIG-1), type I interferon (IFN-α), and type II interferon (IFN-γ). This is the first study to report avian immunological gene expression profiles following an arboviral infection.

Original languageEnglish
Pages (from-to)439-446
Number of pages8
JournalVector-Borne and Zoonotic Diseases
Volume14
Issue number6
DOIs
StatePublished - Jun 1 2014

Fingerprint

Togaviridae
Sparrows
Alphavirus
Pattern Recognition Receptors
Interferons
Birds
Up-Regulation
Viruses
Interferon-gamma
Skeletal Muscle
Spleen
Arboviruses
Interferon Type I
Viremia
Viral RNA
Virus Diseases
Infection
Transcriptome
Myocardium
Gene Expression

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Microbiology
  • Virology
  • Medicine(all)

Cite this

Buggy creek virus (Togaviridae : Alphavirus) upregulates expression of pattern recognition receptors and interferons in house sparrows (Passer domesticus). / Fassbinder-Orth, Carol; Barak, Virginia A.; Rainwater, Ellecia L.; Altrichter, Ashley M.

In: Vector-Borne and Zoonotic Diseases, Vol. 14, No. 6, 01.06.2014, p. 439-446.

Research output: Contribution to journalArticle

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