Abstract
Messages for subunits of voltage-gated calcium channels were examined in the cochlea of the CBA(J) mouse by PCR analysis. Total RNA was extracted from the auditory organs of 16-18-day-old animals. After reverse transcription, resulting cDNA was amplified by PCR with primers targeted to nucleotide sequences corresponding to 12 different calcium channel subunits. PCR products representing subunit gene expression were strongly and consistently amplified for α(1C), α(1D), α(1E), α2δ, β1, β3, and β4 but not for α(1A), α(1B), α(1S), β2, γ. The chosen primers amplified cochlear cDNA to yield an overall pattern of bands different from that of any tissue studied thus far, in particular with respect to the α2δ and β1 subunits; the α2δ product was found to be significantly shorter than the corresponding brain and skeletal muscle isoforms. Nucleotide sequencing confirmed the identity of mouse cochlear subunit cDNAs. The results suggest that L-type and presumptive R-type calcium channels are expressed in the mammalian cochlea and that the α2δ subunits may be coded by a characteristic splice-variant mRNA.
| Original language | English |
|---|---|
| Pages (from-to) | 37-45 |
| Number of pages | 9 |
| Journal | Journal of Neurochemistry |
| Volume | 67 |
| Issue number | 1 |
| State | Published - Jul 1996 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Cellular and Molecular Neuroscience
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Calcium channel subunits in the mouse cochlea. / Green, Glenn E.; Khan, Khalid M.; Beisel, Kirk; Drescher, Marian J.; Hatfield, James S.; Drescher, Dennis G.
In: Journal of Neurochemistry, Vol. 67, No. 1, 07.1996, p. 37-45.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calcium channel subunits in the mouse cochlea
AU - Green, Glenn E.
AU - Khan, Khalid M.
AU - Beisel, Kirk
AU - Drescher, Marian J.
AU - Hatfield, James S.
AU - Drescher, Dennis G.
PY - 1996/7
Y1 - 1996/7
N2 - Messages for subunits of voltage-gated calcium channels were examined in the cochlea of the CBA(J) mouse by PCR analysis. Total RNA was extracted from the auditory organs of 16-18-day-old animals. After reverse transcription, resulting cDNA was amplified by PCR with primers targeted to nucleotide sequences corresponding to 12 different calcium channel subunits. PCR products representing subunit gene expression were strongly and consistently amplified for α(1C), α(1D), α(1E), α2δ, β1, β3, and β4 but not for α(1A), α(1B), α(1S), β2, γ. The chosen primers amplified cochlear cDNA to yield an overall pattern of bands different from that of any tissue studied thus far, in particular with respect to the α2δ and β1 subunits; the α2δ product was found to be significantly shorter than the corresponding brain and skeletal muscle isoforms. Nucleotide sequencing confirmed the identity of mouse cochlear subunit cDNAs. The results suggest that L-type and presumptive R-type calcium channels are expressed in the mammalian cochlea and that the α2δ subunits may be coded by a characteristic splice-variant mRNA.
AB - Messages for subunits of voltage-gated calcium channels were examined in the cochlea of the CBA(J) mouse by PCR analysis. Total RNA was extracted from the auditory organs of 16-18-day-old animals. After reverse transcription, resulting cDNA was amplified by PCR with primers targeted to nucleotide sequences corresponding to 12 different calcium channel subunits. PCR products representing subunit gene expression were strongly and consistently amplified for α(1C), α(1D), α(1E), α2δ, β1, β3, and β4 but not for α(1A), α(1B), α(1S), β2, γ. The chosen primers amplified cochlear cDNA to yield an overall pattern of bands different from that of any tissue studied thus far, in particular with respect to the α2δ and β1 subunits; the α2δ product was found to be significantly shorter than the corresponding brain and skeletal muscle isoforms. Nucleotide sequencing confirmed the identity of mouse cochlear subunit cDNAs. The results suggest that L-type and presumptive R-type calcium channels are expressed in the mammalian cochlea and that the α2δ subunits may be coded by a characteristic splice-variant mRNA.
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M3 - Article
C2 - 8667015
AN - SCOPUS:0030006508
VL - 67
SP - 37
EP - 45
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 1
ER -