Abstract
Vascular smooth muscle cells (VSMCs) play a major role in the development of atherosclerotic and restenotic lesions. The apoptotic process has been implicated in the development of this pathology. In this study, we characterized the induction of apoptosis by calphostin C (CC), a protein kinase C (PKC) inhibitor, in primary human coronary artery smooth muscle cells in the presence and absence of insulin-like growth factor-I (IGF-I). Additionally, we investigated the signal transduction pathways important for IGF-I mediated protection. Calphostin C induced apoptosis, as measured by terminal deoxy-UTP nick-end labeling (TUNEL), in a time- and dose-dependent manner, approaching 20% within 6 h of 50 nM calphostin C treatment. The amount of apoptosis increased to 44.58±8.08%, 47.54±1.66% and 78.1±11.9% after 8, 10 and 12 h of treatment, respectively (p
| Original language | English |
|---|---|
| Pages (from-to) | 1751-1759 |
| Number of pages | 9 |
| Journal | International Immunopharmacology |
| Volume | 3 |
| Issue number | 13-14 |
| DOIs | |
| State | Published - Dec 2003 |
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All Science Journal Classification (ASJC) codes
- Immunology
- Pharmacology
Cite this
Calphostin C as a rapid and strong inducer of apoptosis in human coronary artery smooth muscle cells. / Krueger, Kristopher D.; Hunter, William J.; Del Core, Michael; Agrawal, Devendra K.
In: International Immunopharmacology, Vol. 3, No. 13-14, 12.2003, p. 1751-1759.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Calphostin C as a rapid and strong inducer of apoptosis in human coronary artery smooth muscle cells
AU - Krueger, Kristopher D.
AU - Hunter, William J.
AU - Del Core, Michael
AU - Agrawal, Devendra K.
PY - 2003/12
Y1 - 2003/12
N2 - Vascular smooth muscle cells (VSMCs) play a major role in the development of atherosclerotic and restenotic lesions. The apoptotic process has been implicated in the development of this pathology. In this study, we characterized the induction of apoptosis by calphostin C (CC), a protein kinase C (PKC) inhibitor, in primary human coronary artery smooth muscle cells in the presence and absence of insulin-like growth factor-I (IGF-I). Additionally, we investigated the signal transduction pathways important for IGF-I mediated protection. Calphostin C induced apoptosis, as measured by terminal deoxy-UTP nick-end labeling (TUNEL), in a time- and dose-dependent manner, approaching 20% within 6 h of 50 nM calphostin C treatment. The amount of apoptosis increased to 44.58±8.08%, 47.54±1.66% and 78.1±11.9% after 8, 10 and 12 h of treatment, respectively (p
AB - Vascular smooth muscle cells (VSMCs) play a major role in the development of atherosclerotic and restenotic lesions. The apoptotic process has been implicated in the development of this pathology. In this study, we characterized the induction of apoptosis by calphostin C (CC), a protein kinase C (PKC) inhibitor, in primary human coronary artery smooth muscle cells in the presence and absence of insulin-like growth factor-I (IGF-I). Additionally, we investigated the signal transduction pathways important for IGF-I mediated protection. Calphostin C induced apoptosis, as measured by terminal deoxy-UTP nick-end labeling (TUNEL), in a time- and dose-dependent manner, approaching 20% within 6 h of 50 nM calphostin C treatment. The amount of apoptosis increased to 44.58±8.08%, 47.54±1.66% and 78.1±11.9% after 8, 10 and 12 h of treatment, respectively (p
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U2 - 10.1016/S1567-5769(03)00206-6
DO - 10.1016/S1567-5769(03)00206-6
M3 - Article
VL - 3
SP - 1751
EP - 1759
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 13-14
ER -