Calphostin C as a rapid and strong inducer of apoptosis in human coronary artery smooth muscle cells

Kristopher D. Krueger, William J. Hunter, Michael G. DelCore, Devendra K. Agrawal

Research output: Contribution to journalArticle

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Abstract

Vascular smooth muscle cells (VSMCs) play a major role in the development of atherosclerotic and restenotic lesions. The apoptotic process has been implicated in the development of this pathology. In this study, we characterized the induction of apoptosis by calphostin C (CC), a protein kinase C (PKC) inhibitor, in primary human coronary artery smooth muscle cells in the presence and absence of insulin-like growth factor-I (IGF-I). Additionally, we investigated the signal transduction pathways important for IGF-I mediated protection. Calphostin C induced apoptosis, as measured by terminal deoxy-UTP nick-end labeling (TUNEL), in a time- and dose-dependent manner, approaching 20% within 6 h of 50 nM calphostin C treatment. The amount of apoptosis increased to 44.58±8.08%, 47.54±1.66% and 78.1±11.9% after 8, 10 and 12 h of treatment, respectively (p

Original languageEnglish (US)
Pages (from-to)1751-1759
Number of pages9
JournalInternational Immunopharmacology
Volume3
Issue number13-14
DOIs
StatePublished - Dec 2003

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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