Abstract
CATH.a cells are a catecholaminergic cell line of neuronal origin. The opioid receptor complement expressed by CATH.a cells was defined pharmacologically and by reverse transcription-polymerase chain reaction (RT- PCR). CATH.a cells were found to express mRNA encoding all three of the major subtypes of opioid receptors. The relative abundance of CATH.a cell opioid receptor transcripts was δ > κ > μ. Pharmacological and functional data were in agreement with the results of RT-PCR inasmuch as δ-opioid receptor was identified as the most abundant opioid receptor subtype expressed by CATH.a cells. In addition, at least one of the opioid signalling pathways, inhibition of adenylyl cyclase activity, was found to be operant in this cell line. CATH.a cells should be of general utility for the study of opioid receptor signalling mechanisms in the context of catecholaminergic neurons.
| Original language | English |
|---|---|
| Pages (from-to) | 85-93 |
| Number of pages | 9 |
| Journal | European Journal of Pharmacology |
| Volume | 348 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 1 1998 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Cellular and Molecular Neuroscience
- Pharmacology
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Catecholaminergic CATH.a cells express predominantly δ-opioid receptors. / Bouvier, Claudia; Avram, Dorina; Peterson, Valerie J.; Hettinger, Barbara; Soderstrom, Ken; Murray, Thomas F.; Leid, Mark.
In: European Journal of Pharmacology, Vol. 348, No. 1, 01.05.1998, p. 85-93.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Catecholaminergic CATH.a cells express predominantly δ-opioid receptors
AU - Bouvier, Claudia
AU - Avram, Dorina
AU - Peterson, Valerie J.
AU - Hettinger, Barbara
AU - Soderstrom, Ken
AU - Murray, Thomas F.
AU - Leid, Mark
PY - 1998/5/1
Y1 - 1998/5/1
N2 - CATH.a cells are a catecholaminergic cell line of neuronal origin. The opioid receptor complement expressed by CATH.a cells was defined pharmacologically and by reverse transcription-polymerase chain reaction (RT- PCR). CATH.a cells were found to express mRNA encoding all three of the major subtypes of opioid receptors. The relative abundance of CATH.a cell opioid receptor transcripts was δ > κ > μ. Pharmacological and functional data were in agreement with the results of RT-PCR inasmuch as δ-opioid receptor was identified as the most abundant opioid receptor subtype expressed by CATH.a cells. In addition, at least one of the opioid signalling pathways, inhibition of adenylyl cyclase activity, was found to be operant in this cell line. CATH.a cells should be of general utility for the study of opioid receptor signalling mechanisms in the context of catecholaminergic neurons.
AB - CATH.a cells are a catecholaminergic cell line of neuronal origin. The opioid receptor complement expressed by CATH.a cells was defined pharmacologically and by reverse transcription-polymerase chain reaction (RT- PCR). CATH.a cells were found to express mRNA encoding all three of the major subtypes of opioid receptors. The relative abundance of CATH.a cell opioid receptor transcripts was δ > κ > μ. Pharmacological and functional data were in agreement with the results of RT-PCR inasmuch as δ-opioid receptor was identified as the most abundant opioid receptor subtype expressed by CATH.a cells. In addition, at least one of the opioid signalling pathways, inhibition of adenylyl cyclase activity, was found to be operant in this cell line. CATH.a cells should be of general utility for the study of opioid receptor signalling mechanisms in the context of catecholaminergic neurons.
UR - http://www.scopus.com/inward/record.url?scp=0032077872&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032077872&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(98)00132-0
DO - 10.1016/S0014-2999(98)00132-0
M3 - Article
VL - 348
SP - 85
EP - 93
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -