TY - JOUR
T1 - Catecholaminergic CATH.a cells express predominantly δ-opioid receptors
AU - Bouvier, Claudia
AU - Avram, Dorina
AU - Peterson, Valerie J.
AU - Hettinger, Barbara
AU - Soderstrom, Ken
AU - Murray, Thomas F.
AU - Leid, Mark
N1 - Funding Information:
We thank Drs. D. Chikaraishi for CATH.a cells, David Grandy for CHOμ, CHOκ and CHOδ cell lines, and Jane Ishmael for advice and critical reading of this manuscript. The technical assistance of Beth Olenchek is gratefully acknowledged. This work was funded by NIH grant DA10610 to ML.
PY - 1998/5/1
Y1 - 1998/5/1
N2 - CATH.a cells are a catecholaminergic cell line of neuronal origin. The opioid receptor complement expressed by CATH.a cells was defined pharmacologically and by reverse transcription-polymerase chain reaction (RT- PCR). CATH.a cells were found to express mRNA encoding all three of the major subtypes of opioid receptors. The relative abundance of CATH.a cell opioid receptor transcripts was δ > κ > μ. Pharmacological and functional data were in agreement with the results of RT-PCR inasmuch as δ-opioid receptor was identified as the most abundant opioid receptor subtype expressed by CATH.a cells. In addition, at least one of the opioid signalling pathways, inhibition of adenylyl cyclase activity, was found to be operant in this cell line. CATH.a cells should be of general utility for the study of opioid receptor signalling mechanisms in the context of catecholaminergic neurons.
AB - CATH.a cells are a catecholaminergic cell line of neuronal origin. The opioid receptor complement expressed by CATH.a cells was defined pharmacologically and by reverse transcription-polymerase chain reaction (RT- PCR). CATH.a cells were found to express mRNA encoding all three of the major subtypes of opioid receptors. The relative abundance of CATH.a cell opioid receptor transcripts was δ > κ > μ. Pharmacological and functional data were in agreement with the results of RT-PCR inasmuch as δ-opioid receptor was identified as the most abundant opioid receptor subtype expressed by CATH.a cells. In addition, at least one of the opioid signalling pathways, inhibition of adenylyl cyclase activity, was found to be operant in this cell line. CATH.a cells should be of general utility for the study of opioid receptor signalling mechanisms in the context of catecholaminergic neurons.
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U2 - 10.1016/S0014-2999(98)00132-0
DO - 10.1016/S0014-2999(98)00132-0
M3 - Article
C2 - 9650835
AN - SCOPUS:0032077872
VL - 348
SP - 85
EP - 93
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -