Characterization of a functional neuropeptide F receptor from Drosophila melanogaster

Stephen F. Garczynski, Mark R. Brown, Ping Shen, Thomas F. Murray, Joe W. Crim

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.

Original languageEnglish
Pages (from-to)773-780
Number of pages8
JournalPeptides
Volume23
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Neuropeptide Receptors
Invertebrates
Drosophila melanogaster
Inhibitory Concentration 50
Lymnaea
CHO Cells
Colforsin
Adenylyl Cyclases
Drosophila
In Situ Hybridization
Larva
Vertebrates
Assays
Genes
Genome
Databases
RNA
neuropeptide F
Drosophila neuropeptide F

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Characterization of a functional neuropeptide F receptor from Drosophila melanogaster. / Garczynski, Stephen F.; Brown, Mark R.; Shen, Ping; Murray, Thomas F.; Crim, Joe W.

In: Peptides, Vol. 23, No. 4, 2002, p. 773-780.

Research output: Contribution to journalArticle

Garczynski, Stephen F. ; Brown, Mark R. ; Shen, Ping ; Murray, Thomas F. ; Crim, Joe W. / Characterization of a functional neuropeptide F receptor from Drosophila melanogaster. In: Peptides. 2002 ; Vol. 23, No. 4. pp. 773-780.
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