Abstract
Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
| Original language | English |
|---|---|
| Pages (from-to) | 773-780 |
| Number of pages | 8 |
| Journal | Peptides |
| Volume | 23 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
Characterization of a functional neuropeptide F receptor from Drosophila melanogaster. / Garczynski, Stephen F.; Brown, Mark R.; Shen, Ping; Murray, Thomas F.; Crim, Joe W.
In: Peptides, Vol. 23, No. 4, 2002, p. 773-780.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of a functional neuropeptide F receptor from Drosophila melanogaster
AU - Garczynski, Stephen F.
AU - Brown, Mark R.
AU - Shen, Ping
AU - Murray, Thomas F.
AU - Crim, Joe W.
PY - 2002
Y1 - 2002
N2 - Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
AB - Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036129531&partnerID=8YFLogxK
U2 - 10.1016/S0196-9781(01)00647-7
DO - 10.1016/S0196-9781(01)00647-7
M3 - Article
C2 - 11897397
AN - SCOPUS:0036129531
VL - 23
SP - 773
EP - 780
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 4
ER -