Abstract
Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
Original language | English (US) |
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Pages (from-to) | 773-780 |
Number of pages | 8 |
Journal | Peptides |
Volume | 23 |
Issue number | 4 |
DOIs | |
State | Published - 2002 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Physiology
- Endocrinology
- Cellular and Molecular Neuroscience