Characterization of a functional neuropeptide F receptor from Drosophila melanogaster

Stephen F. Garczynski; Mark R. Brown; Ping Shen; Thomas F. Murray; Joe W. Crim

doi:10.1016/S0196-9781(01)00647-7

Characterization of a functional neuropeptide F receptor from Drosophila melanogaster

Stephen F. Garczynski, Mark R. Brown, Ping Shen, Thomas F. Murray, Joe W. Crim

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced ¹²⁵I-NPF in a concentration-dependent manner (IC₅₀ = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC₅₀ = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.

Original language	English (US)
Pages (from-to)	773-780
Number of pages	8
Journal	Peptides
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/S0196-9781(01)00647-7
State	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/S0196-9781(01)00647-7

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title = "Characterization of a functional neuropeptide F receptor from Drosophila melanogaster",

abstract = "Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.",

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AU - Shen, Ping

AU - Murray, Thomas F.

AU - Crim, Joe W.

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