Characterization of a functional neuropeptide F receptor from Drosophila melanogaster

Stephen F. Garczynski, Mark R. Brown, Ping Shen, Thomas F. Murray, Joe W. Crim

Research output: Contribution to journalArticle

117 Scopus citations

Abstract

Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC50 = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC50 = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.

Original languageEnglish (US)
Pages (from-to)773-780
Number of pages8
JournalPeptides
Volume23
Issue number4
DOIs
StatePublished - Mar 27 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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